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Molecular characterization and complete genome of a novel nepovirus from red clover
I. Koloniuk, J. Přibylová, J. Fránová,
Language English Country Austria
Document type Journal Article
NLK
ProQuest Central
from 2002-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2000-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2002-01-01 to 1 year ago
Public Health Database (ProQuest)
from 2002-01-01 to 1 year ago
- MeSH
- Phylogeny MeSH
- Genome, Viral * MeSH
- Plant Diseases virology MeSH
- Nepovirus classification genetics isolation & purification MeSH
- RNA, Viral genetics MeSH
- Trifolium virology MeSH
- Viral Proteins genetics MeSH
- High-Throughput Nucleotide Sequencing MeSH
- Publication type
- Journal Article MeSH
During high throughput sequencing (HTS) of leaves from a symptomatic red clover plant, a new RNA virus, tentatively named red clover nepovirus A (RCNVA), was discovered. The complete genomic sequence was determined and characterized. Particularly noteworthy was that RCNVA shares high sequence identities in RNA1 with a group of phylogenetically related nepoviruses while homologies in the RNA2 segments are markedly lower. Based on the genomic organization and phylogenetic attributes, RCNVA should be classified as a novel virus of the genus Nepovirus (subfamily Comovirinae, family Secoviridae, order Picornavirales).
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- $a During high throughput sequencing (HTS) of leaves from a symptomatic red clover plant, a new RNA virus, tentatively named red clover nepovirus A (RCNVA), was discovered. The complete genomic sequence was determined and characterized. Particularly noteworthy was that RCNVA shares high sequence identities in RNA1 with a group of phylogenetically related nepoviruses while homologies in the RNA2 segments are markedly lower. Based on the genomic organization and phylogenetic attributes, RCNVA should be classified as a novel virus of the genus Nepovirus (subfamily Comovirinae, family Secoviridae, order Picornavirales).
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- $a Fránová, Jana $u Department of Plant Virology, Biology Centre, Institute of Plant Molecular Biology, The Czech Academy of Sciences, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic.
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