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WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity
T. Prát, J. Hajný, W. Grunewald, M. Vasileva, G. Molnár, R. Tejos, M. Schmid, M. Sauer, J. Friml,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
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- MeSH
- Arabidopsis genetics growth & development MeSH
- Plants, Genetically Modified MeSH
- Gene Regulatory Networks * drug effects MeSH
- Plant Roots drug effects genetics growth & development metabolism MeSH
- Indoleacetic Acids metabolism pharmacology MeSH
- Membrane Transport Proteins genetics metabolism MeSH
- Microarray Analysis MeSH
- Cell Polarity * genetics MeSH
- Arabidopsis Proteins genetics metabolism physiology MeSH
- Gene Expression Regulation, Plant drug effects MeSH
- Gene Expression Profiling MeSH
- Transcription Factors physiology MeSH
- Feedback, Physiological drug effects MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17- and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain- and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development.
Department of Plant Physiology University of Potsdam Potsdam Germany
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