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Single cell genomics of uncultured marine alveolates shows paraphyly of basal dinoflagellates

JFH. Strassert, A. Karnkowska, E. Hehenberger, J. Del Campo, M. Kolisko, N. Okamoto, F. Burki, J. Janouškovec, C. Poirier, G. Leonard, SJ. Hallam, TA. Richards, AZ. Worden, AE. Santoro, PJ. Keeling,

. 2018 ; 12 (1) : 304-308. [pub] 20171010

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc18024584

Marine alveolates (MALVs) are diverse and widespread early-branching dinoflagellates, but most knowledge of the group comes from a few cultured species that are generally not abundant in natural samples, or from diversity analyses of PCR-based environmental SSU rRNA gene sequences. To more broadly examine MALV genomes, we generated single cell genome sequences from seven individually isolated cells. Genes expected of heterotrophic eukaryotes were found, with interesting exceptions like presence of proteorhodopsin and vacuolar H+-pyrophosphatase. Phylogenetic analysis of concatenated SSU and LSU rRNA gene sequences provided strong support for the paraphyly of MALV lineages. Dinoflagellate viral nucleoproteins were found only in MALV groups that branched as sister to dinokaryotes. Our findings indicate that multiple independent origins of several characteristics early in dinoflagellate evolution, such as a parasitic life style, underlie the environmental diversity of MALVs, and suggest they have more varied trophic modes than previously thought.

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$a Karnkowska, Anna $u Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada.
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$a Hehenberger, Elisabeth $u Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada.
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$a Del Campo, Javier $u Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada.
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$a Kolisko, Martin $u Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada. Institute of Parasitology, Biology Centre CAS, České Budějovice, Czech Republic.
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$a Janouškovec, Jan $u Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada.
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$a Leonard, Guy $u Biosciences, University of Exeter, Exeter, UK.
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