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Rapid Gene Isolation Using MutChromSeq

B. Steuernagel, J. Vrána, M. Karafiátová, BBH. Wulff, J. Doležel,

. 2017 ; 1659 (-) : 231-243.

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc18024696

MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.

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