Human polyomaviruses such as JC polyomavirus and BK polyomavirus have long been well known pathogens of immunocompromised patients. Several new members of this viral family have been described during the last decade. Human polyomavirus 9 seems to be a novel pathogen of transplanted patients according to some studies. The aim of our study was to determine the presence of human polyomavirus 9 in patients after kidney or stem cell transplantation (SCT) at the University Hospital in Hradec Kralove, Czech Republic. Overall 100 patients, 65 after kidney transplantation and 35 after SCT, were included into the study. At least three follow-up samples from each patient were examined for human polyomavirus 9 DNA presentation with the two previously described in-house PCR protocols. Despite the frequent reactivation of human CMV (14.3% in kidney transplantation and 63.3% after SCT) or BK polyomavirus in our patient group, there was no positivity for human polyomavirus 9 either in blood samples or urine samples. One of the possible reasons for this discrepancy versus previous published studies could be a relatively low proportion of patients treated by induction therapy before kidney transplantation in our study cohort.

Department of Epidemiology Faculty of Military Health Science University of Defence Hradec Kralove Czech Republic

Department of Molecular Biology Institute of Clinical Biochemistry and Diagnostics University Hospital Hradec Kralove Czech Republic

Faculty of Medicine in Hradec Kralove Charles University Prague Czech Republic 4th Department of Internal Medicine Haematology Charles University University Hospital Hradec Kralove Czech Republic

Faculty of Medicine in Hradec Kralove Charles University Prague Czech Republic Transplantation Centre of Urological Clinic University Hospital Hradec Kralove Czech Republic

Faculty of Medicine in Hradec Kralove Charles University Prague Czech Republic University Hospital Haemodialysis Centre Hradec Kralove Czech Republic

Institute of Clinical Microbiology University Hospital Hradec Kralove Czech Republic Faculty of Medicine in Hradec Kralove Charles University Prague Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc18024881

003      

CZ-PrNML

005      

20180717100236.0

007      

ta

008      

180709s2017 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1002/jmv.24892 $2 doi

035    __

$a (PubMed)28667764

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxu

100    1_

$a Fajfr, Miroslav $u Institute of Clinical Microbiology, University Hospital, Hradec Kralove, Czech Republic. Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic.

245    10

$a Human polyomavirus 9 in immunocompromised patients in the University Hospital in Hradec Kralove, Czech Republic / $c M. Fajfr, L. Pliskova, R. Kutova, M. Matyskova-Kubisova, P. Navratil, J. Radocha, Z. Valenta, S. Dusilova-Sulkova,

520    9_

$a Human polyomaviruses such as JC polyomavirus and BK polyomavirus have long been well known pathogens of immunocompromised patients. Several new members of this viral family have been described during the last decade. Human polyomavirus 9 seems to be a novel pathogen of transplanted patients according to some studies. The aim of our study was to determine the presence of human polyomavirus 9 in patients after kidney or stem cell transplantation (SCT) at the University Hospital in Hradec Kralove, Czech Republic. Overall 100 patients, 65 after kidney transplantation and 35 after SCT, were included into the study. At least three follow-up samples from each patient were examined for human polyomavirus 9 DNA presentation with the two previously described in-house PCR protocols. Despite the frequent reactivation of human CMV (14.3% in kidney transplantation and 63.3% after SCT) or BK polyomavirus in our patient group, there was no positivity for human polyomavirus 9 either in blood samples or urine samples. One of the possible reasons for this discrepancy versus previous published studies could be a relatively low proportion of patients treated by induction therapy before kidney transplantation in our study cohort.

650    _2

$a dospělí $7 D000328

650    _2

$a senioři $7 D000368

650    _2

$a kohortové studie $7 D015331

650    _2

$a Česká republika $x epidemiologie $7 D018153

650    _2

$a DNA virů $x genetika $7 D004279

650    _2

$a ženské pohlaví $7 D005260

650    _2

$a nemocnice univerzitní $7 D006785

650    _2

$a lidé $7 D006801

650    12

$a hostitel s imunodeficiencí $7 D016867

650    _2

$a transplantace ledvin $x škodlivé účinky $7 D016030

650    _2

$a mužské pohlaví $7 D008297

650    _2

$a lidé středního věku $7 D008875

650    _2

$a polymerázová řetězová reakce $7 D016133

650    _2

$a Polyomaviridae $x genetika $x izolace a purifikace $x patogenita $7 D027382

650    _2

$a polyomavirové infekce $x epidemiologie $x virologie $7 D027601

650    _2

$a transplantace kmenových buněk $x škodlivé účinky $7 D033581

650    _2

$a mladý dospělý $7 D055815

655    _2

$a časopisecké články $7 D016428

700    1_

$a Pliskova, Lenka $u Department of Molecular Biology, Institute of Clinical Biochemistry and Diagnostics, University Hospital, Hradec Kralove, Czech Republic.

700    1_

$a Kutova, Radka $u Department of Molecular Biology, Institute of Clinical Biochemistry and Diagnostics, University Hospital, Hradec Kralove, Czech Republic.

700    1_

$a Matyskova-Kubisova, Michaela $u Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic. University Hospital, Haemodialysis Centre, Hradec Kralove, Czech Republic.

700    1_

$a Navratil, Pavel $u Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic. Transplantation Centre of Urological Clinic, University Hospital, Hradec Kralove, Czech Republic.

700    1_

$a Radocha, Jakub $u Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic. 4th Department of Internal Medicine-Haematology, Charles University, University Hospital, Hradec Kralove, Czech Republic.

700    1_

$a Valenta, Zbynek $u Department of Epidemiology, Faculty of Military Health Science, University of Defence, Hradec Kralove, Czech Republic.

700    1_

$a Dusilova-Sulkova, Sylvie $u Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic. University Hospital, Haemodialysis Centre, Hradec Kralove, Czech Republic.

773    0_

$w MED00002794 $t Journal of medical virology $x 1096-9071 $g Roč. 89, č. 12 (2017), s. 2230-2234

856    41

$u https://pubmed.ncbi.nlm.nih.gov/28667764 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20180709 $b ABA008

991    __

$a 20180717100535 $b ABA008

999    __

$a ok $b bmc $g 1317012 $s 1021802

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2017 $b 89 $c 12 $d 2230-2234 $e 20170905 $i 1096-9071 $m Journal of medical virology $n J Med Virol $x MED00002794

LZP    __

$a Pubmed-20180709