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Trueperella pyogenes isolated from a brain abscess of an adult roebuck (Capreolus capreolus)
JP. Wickhorst, AA. Hassan, OH. Sheet, T. Eisenberg, O. Sammra, M. Alssahen, C. Lämmler, E. Prenger-Berninghoff, M. Zschöck, M. Timke, A. Abdulmawjood,
Language English Country United States
Document type Journal Article
- MeSH
- Brain Abscess microbiology veterinary MeSH
- Actinomycetaceae classification genetics isolation & purification physiology MeSH
- Bacterial Proteins genetics metabolism MeSH
- Virulence Factors genetics metabolism MeSH
- Phylogeny MeSH
- Actinomycetales Infections microbiology veterinary MeSH
- Deer MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rRNA intergenic spacer region (ISR); by sequencing the target genes rpoB, gap, and tuf; and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. The T. pyogenes strain could additionally be characterized by PCR-mediated amplification of several known and putative virulence factor-encoding genes which revealed the presence of the genes plo encoding pyolysin and nanH and nanP encoding neuraminidases; the genes fimA, fimC, and fimE encoding the fimbrial subunits FimA, FimC, and FimE; and the gene cbpA encoding collagen-binding protein CbpA. The present data give a detailed characterization of a T. pyogenes strain isolated from a brain abscess of a roebuck. However, the route of infection of the roebuck remains unclear.
Department of Veterinary Public Health College of Veterinary Medicine University of Mosul Mosul Iraq
Entwicklung Bioanalyse Bruker Daltonik GmbH Fahrenheitstraße 4 28359 Bremen Germany
Landesbetrieb Hessisches Landeslabor Schubertstraße 60 35392 Giessen Germany
References provided by Crossref.org
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