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Ovarian steroid hormone secretion activity examined after supplementation of green tea extract
S. Roychoudhury, M. Halenar, V. Tupa, K. Michalcova, S. Nath, M. Kacaniova, A. Kolesarova
Language English Country Czech Republic
Document type Journal Article
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- MeSH
- Camellia sinensis * MeSH
- Estradiol metabolism secretion MeSH
- Granulosa Cells drug effects metabolism secretion MeSH
- Follicle Stimulating Hormone pharmacology MeSH
- Phytotherapy MeSH
- Cells, Cultured MeSH
- Plants, Medicinal MeSH
- Progesterone metabolism secretion MeSH
- Plant Extracts isolation & purification pharmacology MeSH
- Sus scrofa MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
This study aimed at examining the secretion activity of steroid hormones progesterone and 17beta-estradiol by porcine ovarian granulosa cells after addition of green tea extract. Granulosa cells were incubated with green tea extract (at doses of 0.01, 0.1, 1, 10 and 100 microg.ml(-1). Another set of cells were incubated with green tea extract at the above doses along with additional supplementation of follicle stimulating hormone (FSH) at 10 microg.ml(-1). Release of hormones by granulosa cells was assessed by EIA after 24 h exposure. Secretion of steroid hormones was not affected either by green tea extract alone or after FSH supplementation with green tea extract. Results indicate that ovarian steroidogenesis is not affected by green tea under conditions used in the experiment.
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- $a This study aimed at examining the secretion activity of steroid hormones progesterone and 17beta-estradiol by porcine ovarian granulosa cells after addition of green tea extract. Granulosa cells were incubated with green tea extract (at doses of 0.01, 0.1, 1, 10 and 100 microg.ml(-1). Another set of cells were incubated with green tea extract at the above doses along with additional supplementation of follicle stimulating hormone (FSH) at 10 microg.ml(-1). Release of hormones by granulosa cells was assessed by EIA after 24 h exposure. Secretion of steroid hormones was not affected either by green tea extract alone or after FSH supplementation with green tea extract. Results indicate that ovarian steroidogenesis is not affected by green tea under conditions used in the experiment.
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