• Je něco špatně v tomto záznamu ?

Determination of oxycodone and its major metabolites in haematic and urinary matrices: Comparison of traditional and miniaturised sampling approaches

M. Protti, MC. Catapano, BG. Samolsky Dekel, J. Rudge, G. Gerra, L. Somaini, R. Mandrioli, L. Mercolini,

. 2018 ; 152 (-) : 204-214. [pub] 20180131

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18033336

Oxycodone is a widely prescribed, full agonist opioid analgesic. As such, it is used clinically to treat different kinds of painful conditions, with a relatively high potential for doping practices in athletes. In this paper, different classic and innovative miniaturised matrices from blood and urine have been studied and compared, to evaluate their relative merits and drawbacks within therapeutic drug monitoring (TDM) and to implement new protocols for anti-doping analysis. Plasma, dried blood spots (DBS) and dried plasma spots (DPS) have been studied for TDM purposes, while urine, dried urine spots (DUS) and volumetric absorptive microsamples (VAMS) from urine for anti-doping. These sampling techniques were coupled to an original bioanalytical method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the evaluation and monitoring of the levels of oxycodone and its major metabolites (noroxycodone and oxymorphone) in patients under pain management and in athletes. The method was validated according to international guidelines, with good results in terms of precision, extraction yield and accuracy for all considered micromatrices. Thus, the proposed sampling, pre-treatment and analysis are attractive strategies for oxycodone determination in human blood and urine, with advanced options for application to derived micromatrices. Microsampling procedures have significant advantages over classic biological matrices like simplified sampling, storage and processing, but also in terms of precision (<9.0% for DBS, <7.7% for DPS, <7.1% for DUS, <5.3% for VAMS) and accuracy (>73% for DBS, >78% for DPS, >74% for DUS, >78% for VAMS). As regards extraction yield, traditional and miniaturised sampling approaches are comparable (>67% for DBS, >74% for DPS, >75% for DUS, >75% for VAMS). All dried matrices have very low volumes, leading to a significant advantage in terms of analysis feasibility. On the other hand, this also leads to a corresponding decrease in the overall sensitivity.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18033336
003      
CZ-PrNML
005      
20181009110306.0
007      
ta
008      
181008s2018 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.jpba.2018.01.043 $2 doi
035    __
$a (PubMed)29414014
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Protti, Michele $u Pharmaco-Toxicological Analysis Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, Bologna, Italy. Electronic address: michele.protti2@unibo.it.
245    10
$a Determination of oxycodone and its major metabolites in haematic and urinary matrices: Comparison of traditional and miniaturised sampling approaches / $c M. Protti, MC. Catapano, BG. Samolsky Dekel, J. Rudge, G. Gerra, L. Somaini, R. Mandrioli, L. Mercolini,
520    9_
$a Oxycodone is a widely prescribed, full agonist opioid analgesic. As such, it is used clinically to treat different kinds of painful conditions, with a relatively high potential for doping practices in athletes. In this paper, different classic and innovative miniaturised matrices from blood and urine have been studied and compared, to evaluate their relative merits and drawbacks within therapeutic drug monitoring (TDM) and to implement new protocols for anti-doping analysis. Plasma, dried blood spots (DBS) and dried plasma spots (DPS) have been studied for TDM purposes, while urine, dried urine spots (DUS) and volumetric absorptive microsamples (VAMS) from urine for anti-doping. These sampling techniques were coupled to an original bioanalytical method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the evaluation and monitoring of the levels of oxycodone and its major metabolites (noroxycodone and oxymorphone) in patients under pain management and in athletes. The method was validated according to international guidelines, with good results in terms of precision, extraction yield and accuracy for all considered micromatrices. Thus, the proposed sampling, pre-treatment and analysis are attractive strategies for oxycodone determination in human blood and urine, with advanced options for application to derived micromatrices. Microsampling procedures have significant advantages over classic biological matrices like simplified sampling, storage and processing, but also in terms of precision (<9.0% for DBS, <7.7% for DPS, <7.1% for DUS, <5.3% for VAMS) and accuracy (>73% for DBS, >78% for DPS, >74% for DUS, >78% for VAMS). As regards extraction yield, traditional and miniaturised sampling approaches are comparable (>67% for DBS, >74% for DPS, >75% for DUS, >75% for VAMS). All dried matrices have very low volumes, leading to a significant advantage in terms of analysis feasibility. On the other hand, this also leads to a corresponding decrease in the overall sensitivity.
650    _2
$a odběr vzorku krve $7 D001800
650    _2
$a tělesné tekutiny $x chemie $7 D001826
650    _2
$a chromatografie kapalinová $x metody $7 D002853
650    _2
$a doping ve sportu $x metody $7 D004300
650    _2
$a test suché kapky krve $x metody $7 D059788
650    _2
$a monitorování léčiv $x metody $7 D016903
650    _2
$a lidé $7 D006801
650    _2
$a miniaturizace $x metody $7 D008904
650    _2
$a morfinany $x krev $x moč $7 D009019
650    _2
$a oxykodon $x krev $x moč $7 D010098
650    _2
$a oxymorfon $x krev $x moč $7 D010111
650    _2
$a krevní plazma $x chemie $7 D010949
650    _2
$a odběr biologického vzorku $x metody $7 D013048
650    _2
$a tandemová hmotnostní spektrometrie $x metody $7 D053719
650    _2
$a moč $x chemie $7 D014556
655    _2
$a časopisecké články $7 D016428
700    1_
$a Catapano, Maria Carmen $u Pharmaco-Toxicological Analysis Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, Bologna, Italy; Department of Pharmacology and Toxicology, Charles University, Hradec Králové, Czech Republic.
700    1_
$a Samolsky Dekel, Boaz Gedaliahu $u Department of Medicine and Surgery Sciences, Alma Mater Studiorum - University of Bologna, Bologna, Italy.
700    1_
$a Rudge, James $u Neoteryx LLC, Torrance, CA, USA.
700    1_
$a Gerra, Gilberto $u Drug Prevention and Health Branch, Division for Operations, United Nation Office on Drugs and Crime, Vienna, Austria.
700    1_
$a Somaini, Lorenzo $u Addiction Treatment Centre, Local Health Service, Cossato, Biella, Italy.
700    1_
$a Mandrioli, Roberto $u Department for Life Quality Studies, Alma Mater Studiorum - University of Bologna, Rimini, Italy.
700    1_
$a Mercolini, Laura $u Pharmaco-Toxicological Analysis Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, Bologna, Italy.
773    0_
$w MED00002894 $t Journal of pharmaceutical and biomedical analysis $x 1873-264X $g Roč. 152, č. - (2018), s. 204-214
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29414014 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20181008 $b ABA008
991    __
$a 20181009110754 $b ABA008
999    __
$a ok $b bmc $g 1340109 $s 1030330
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 152 $c - $d 204-214 $e 20180131 $i 1873-264X $m Journal of pharmaceutical and biomedical analysis $n J Pharm Biomed Anal $x MED00002894
LZP    __
$a Pubmed-20181008

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...