Ectomycorrhizal (ECM) fungi, as symbionts of many tree species in temperate forests, are thought to play an important role in forest regeneration processes after large disturbances. Their reaction to different disturbance and management regimes was studied in spruce forests (Lariceto-Piceetum) 10 years after a severe windthrow in the Tatra National Park (Slovak Republic). ECM community structure was compared between different "management types″-cleared area (EXT), area affected by wildfire (FIRE), uncleared area left for natural development (NEX), and mature forest as a control (REF). Based on Illumina sequencing of soil samples, we determined that the percentage of sequences assigned to ECM fungi decreased with increasing disturbance and management intensity (REF → NEX → EXT → FIRE). Similarly, the total number of ECM species per each of ten sampling points per plot (100 ha) differed between managed (EXT-11 species, FIRE-9) and unmanaged (NEX-16, REF-14) treatments. On the other hand, the percentage of sequences belonging to ericoid mycorrhizal fungi increased. Management type significantly influenced the composition of the ECM community, while vegetation and soil characteristics explained less data variation. The ECM species assemblage of the unmanaged site (NEX) was the most similar to the mature forest, while that of the burnt site was the most different. Thelephora terrestris dominated in all treatments affected by windthrow, accompanied by Tylospora fibrillosa (NEX) and Tylospora asterophora (EXT and FIRE). Management regime was also the most important factor affecting ECM species composition on the roots of spruce seedlings assessed by Sanger sequencing.

Department of Botany Faculty of Science University of South Bohemia Branišovská 31 37005 České Budějovice Czech Republic Global Change Research Institute Czech Academy of Sciences Lipová 1789 9 37005 České Budějovice Czech Republic

Faculty of Forestry Technical University in Zvolen T G Masaryka 2117 24 960 53 Zvolen Slovakia Research Station of TANAP State Forest of TANAP 059 60 Tatranská Lomnica Slovakia

Global Change Research Institute Czech Academy of Sciences Lipová 1789 9 37005 České Budějovice Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc18033383

003      

CZ-PrNML

005      

20181016120637.0

007      

ta

008      

181008s2018 gw f 000 0|eng||

009      

AR

024    7_

$a 10.1007/s00572-018-0820-5 $2 doi

035    __

$a (PubMed)29352412

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a gw

100    1_

$a Vašutová, Martina $u Department of Botany, Faculty of Science, University of South Bohemia, Branišovská 31, 37005, České Budějovice, Czech Republic. mvasutova@prf.jcu.cz. Global Change Research Institute, Czech Academy of Sciences, Lipová 1789/9, 37005, České Budějovice, Czech Republic. mvasutova@prf.jcu.cz.

245    10

$a Management regime is the most important factor influencing ectomycorrhizal species community in Norway spruce forests after windthrow / $c M. Vašutová, M. Edwards-Jonášová, P. Veselá, L. Effenberková, P. Fleischer, P. Cudlín,

520    9_

$a Ectomycorrhizal (ECM) fungi, as symbionts of many tree species in temperate forests, are thought to play an important role in forest regeneration processes after large disturbances. Their reaction to different disturbance and management regimes was studied in spruce forests (Lariceto-Piceetum) 10 years after a severe windthrow in the Tatra National Park (Slovak Republic). ECM community structure was compared between different "management types″-cleared area (EXT), area affected by wildfire (FIRE), uncleared area left for natural development (NEX), and mature forest as a control (REF). Based on Illumina sequencing of soil samples, we determined that the percentage of sequences assigned to ECM fungi decreased with increasing disturbance and management intensity (REF → NEX → EXT → FIRE). Similarly, the total number of ECM species per each of ten sampling points per plot (100 ha) differed between managed (EXT-11 species, FIRE-9) and unmanaged (NEX-16, REF-14) treatments. On the other hand, the percentage of sequences belonging to ericoid mycorrhizal fungi increased. Management type significantly influenced the composition of the ECM community, while vegetation and soil characteristics explained less data variation. The ECM species assemblage of the unmanaged site (NEX) was the most similar to the mature forest, while that of the burnt site was the most different. Thelephora terrestris dominated in all treatments affected by windthrow, accompanied by Tylospora fibrillosa (NEX) and Tylospora asterophora (EXT and FIRE). Management regime was also the most important factor affecting ECM species composition on the roots of spruce seedlings assessed by Sanger sequencing.

650    _2

$a Basidiomycota $x izolace a purifikace $x fyziologie $7 D001487

650    12

$a biodiverzita $7 D044822

650    _2

$a lesnictví $x metody $7 D016468

650    _2

$a lesy $7 D065928

650    _2

$a mykorhiza $x izolace a purifikace $x fyziologie $7 D038821

650    _2

$a smrk $x mikrobiologie $7 D028222

650    _2

$a semenáček $x mikrobiologie $7 D036226

650    _2

$a sekvenční analýza DNA $7 D017422

650    _2

$a Slovenská republika $7 D018154

650    _2

$a vítr $7 D014919

655    _2

$a časopisecké články $7 D016428

700    1_

$a Edwards-Jonášová, Magda $u Global Change Research Institute, Czech Academy of Sciences, Lipová 1789/9, 37005, České Budějovice, Czech Republic.

700    1_

$a Veselá, Petra $u Global Change Research Institute, Czech Academy of Sciences, Lipová 1789/9, 37005, České Budějovice, Czech Republic.

700    1_

$a Effenberková, Lenka $u Global Change Research Institute, Czech Academy of Sciences, Lipová 1789/9, 37005, České Budějovice, Czech Republic.

700    1_

$a Fleischer, Peter $u Faculty of Forestry, Technical University in Zvolen, T. G. Masaryka 2117/24, 960 53, Zvolen, Slovakia. Research Station of TANAP, State Forest of TANAP, 059 60, Tatranská Lomnica, Slovakia.

700    1_

$a Cudlín, Pavel $u Global Change Research Institute, Czech Academy of Sciences, Lipová 1789/9, 37005, České Budějovice, Czech Republic.

773    0_

$w MED00180395 $t Mycorrhiza $x 1432-1890 $g Roč. 28, č. 3 (2018), s. 221-233

856    41

$u https://pubmed.ncbi.nlm.nih.gov/29352412 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20181008 $b ABA008

991    __

$a 20181016121134 $b ABA008

999    __

$a ok $b bmc $g 1340127 $s 1030377

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2018 $b 28 $c 3 $d 221-233 $e 20180119 $i 1432-1890 $m Mycorrhiza $n Mycorrhiza $x MED00180395

LZP    __

$a Pubmed-20181008