Structural properties of plasmid DNA and model lipid membrane treated with newly synthesized platinum(II) complex cis-[PtCl2{P(CH2CH2COOH)3}2] (cis-DTCEP for short) were studied and compared with effects of anticancer drug cisplatin, cis-[Pt(NH3)2Cl2] (cis-DDP for short). Time Correlated Single Photon Counting Fluorescence Correlation Spectroscopy (TCSPC-FCS) was employed to study interactions between those platinum complexes and DNA. The TCSPC-FCS results suggest that bonding of cis-DTCEP derivative to DNA leads to plasmid strain realignment towards much more compact structure than in the case of cis-DDP. Application of both differential scanning calorimetry and infrared spectroscopy to platinum complexes/DPPC showed that cis-DTCEP slightly increases the phospholipid's main phase transition temperature resulting in decreased fluidity of the model membrane. The newly investigated compound-similarly to cis-DDP-interacts mainly with the DPPC head group however not only by the means of electrostatic forces: this compound probably enters into hydrophilic region of the lipid bilayer and forms hydrogen bonds with COO groups of glycerol and PO2- group of DPPC.

Department of Physics and Biophysics Wrocław University of Environmental and Life Sciences ul Norwida 25 50 375 Wrocław Poland

Department of Physics and Biophysics Wrocław University of Environmental and Life Sciences ul Norwida 25 50 375 Wrocław Poland J Heyrovsky Institute of Physical Chemistry of the ASCR v v i Dolejškova 2155 3 182 23 Prague 8 Czech Republic

J Heyrovsky Institute of Physical Chemistry of the ASCR v v i Dolejškova 2155 3 182 23 Prague 8 Czech Republic

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$a Pruchnik, Hanna $u Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, ul. Norwida 25, 50-375, Wrocław, Poland. hanna.pruchnik@up.wroc.pl.

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$a Interaction of Newly Platinum(II) with Tris(2-carboxyethyl)phosphine Complex with DNA and Model Lipid Membrane / $c H. Pruchnik, T. Kral, M. Hof,

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$a Structural properties of plasmid DNA and model lipid membrane treated with newly synthesized platinum(II) complex cis-[PtCl2{P(CH2CH2COOH)3}2] (cis-DTCEP for short) were studied and compared with effects of anticancer drug cisplatin, cis-[Pt(NH3)2Cl2] (cis-DDP for short). Time Correlated Single Photon Counting Fluorescence Correlation Spectroscopy (TCSPC-FCS) was employed to study interactions between those platinum complexes and DNA. The TCSPC-FCS results suggest that bonding of cis-DTCEP derivative to DNA leads to plasmid strain realignment towards much more compact structure than in the case of cis-DDP. Application of both differential scanning calorimetry and infrared spectroscopy to platinum complexes/DPPC showed that cis-DTCEP slightly increases the phospholipid's main phase transition temperature resulting in decreased fluidity of the model membrane. The newly investigated compound-similarly to cis-DDP-interacts mainly with the DPPC head group however not only by the means of electrostatic forces: this compound probably enters into hydrophilic region of the lipid bilayer and forms hydrogen bonds with COO groups of glycerol and PO2- group of DPPC.

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$a Kral, Teresa $u Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, ul. Norwida 25, 50-375, Wrocław, Poland. J. Heyrovsky Institute of Physical Chemistry of the ASCR, v.v.i., Dolejškova 2155/3, 182 23, Prague 8, Czech Republic.

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$a Hof, Martin $u J. Heyrovsky Institute of Physical Chemistry of the ASCR, v.v.i., Dolejškova 2155/3, 182 23, Prague 8, Czech Republic.

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