Effect of 7-nitroindazole, a neuronal nitric oxide synthase inhibitor, on behavioral and physiological parameters
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24908089
DOI
10.33549/physiolres.932781
PII: 932781
Knihovny.cz E-resources
- MeSH
- Blood Gas Analysis MeSH
- Time Factors MeSH
- Behavior, Animal drug effects MeSH
- Electroencephalography MeSH
- Indazoles pharmacology MeSH
- Enzyme Inhibitors pharmacology MeSH
- Blood Pressure drug effects MeSH
- Evoked Potentials, Motor MeSH
- Motor Cortex drug effects enzymology MeSH
- Exploratory Behavior drug effects MeSH
- Motor Activity drug effects MeSH
- Rats, Wistar MeSH
- Nitric Oxide Synthase Type I antagonists & inhibitors metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 7-nitroindazole MeSH Browser
- Indazoles MeSH
- Enzyme Inhibitors MeSH
- Nos1 protein, rat MeSH Browser
- Nitric Oxide Synthase Type I MeSH
The role of brain derived nitric oxide in the physiology and behavior remains disputable. One of the reasons of the controversies might be systemic side effects of nitric oxide synthase inhibitors. Therefore, under nNOS inhibition by 7-nitroindazole (7-NI) we carried out recordings of blood gasses, blood pressure and spontaneous EEG in conscious adult rats. Locomotion and spontaneous behavior were assessed in an open field. In addition skilled walking and limb coordination were evaluated using a ladder rung walking test. The blood gas analysis revealed a significant increase in pCO(2) 180 min and 240 min after the application of 7-NI. The power and entropy decreased simultaneously with a shift of the mean frequency of the spontaneous EEG toward slow oscillations after 7-NI treatment. The thresholds of evoked potentials underwent a significant drop and a trend towards a slight increase in the I-O curve slope was observed. 7-NI significantly suppressed open field behavior expressed as distance moved, exploratory rearing and grooming. As for the ladder rung walking test the 7-NI treated animals had more errors in foot placement indicating impairment in limb coordination. Therefore our findings suggest that 7-NI increased cortical excitability and altered some physiological and behavioral parameters.
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Epilepsy Research in the Institute of Physiology of the Czech Academy of Sciences in Prague
