• Je něco špatně v tomto záznamu ?

Fluctuations of pol I and fibrillarin contents of the nucleoli

M. Hornáček, L. Kováčik, T. Mazel, D. Cmarko, E. Bártová, I. Raška, E. Smirnov,

. 2017 ; 8 (4) : 421-432. [pub] 20170616

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18033862

Nucleoli are formed on the basis of ribosomal DNA (rDNA) clusters called Nucleolus Organizer Regions (NORs). Each NOR contains multiple genes coding for RNAs of the ribosomal particles. The prominent components of the nucleolar ultrastructure, fibrillar centers (FC) and dense fibrillar components (DFC), together compose FC/DFC units. These units are centers of rDNA transcription by RNA polymerase I (pol I), as well as the early processing events, in which an essential role belongs to fibrillarin. Each FC/DFC unit probably corresponds to a single transcriptionally active gene. In this work, we transfected human-derived cells with GFP-RPA43 (subunit of pol I) and RFP-fibrillarin. Following changes of the fluorescent signals in individual FC/DFC units, we found two kinds of kinetics: 1) the rapid fluctuations with periods of 2-3 min, when the pol I and fibrillarin signals oscillated in anti-phase manner, and the intensities of pol I in the neighboring FC/DFC units did not correlate. 2) fluctuations with periods of 10 to 60 min, in which pol I and fibrillarin signals measured in the same unit did not correlate, but pol I signals in the units belonging to different nucleoli were synchronized. Our data indicate that a complex pulsing activity of transcription as well as early processing is common for ribosomal genes.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18033862
003      
CZ-PrNML
005      
20181016095608.0
007      
ta
008      
181008s2017 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1080/19491034.2017.1306160 $2 doi
035    __
$a (PubMed)28622108
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Hornáček, M $u a Institute of Biology and Medical Genetics, First Faculty of Medicine , Charles University, and General University Hospital in Prague , Prague , Czech Republic.
245    10
$a Fluctuations of pol I and fibrillarin contents of the nucleoli / $c M. Hornáček, L. Kováčik, T. Mazel, D. Cmarko, E. Bártová, I. Raška, E. Smirnov,
520    9_
$a Nucleoli are formed on the basis of ribosomal DNA (rDNA) clusters called Nucleolus Organizer Regions (NORs). Each NOR contains multiple genes coding for RNAs of the ribosomal particles. The prominent components of the nucleolar ultrastructure, fibrillar centers (FC) and dense fibrillar components (DFC), together compose FC/DFC units. These units are centers of rDNA transcription by RNA polymerase I (pol I), as well as the early processing events, in which an essential role belongs to fibrillarin. Each FC/DFC unit probably corresponds to a single transcriptionally active gene. In this work, we transfected human-derived cells with GFP-RPA43 (subunit of pol I) and RFP-fibrillarin. Following changes of the fluorescent signals in individual FC/DFC units, we found two kinds of kinetics: 1) the rapid fluctuations with periods of 2-3 min, when the pol I and fibrillarin signals oscillated in anti-phase manner, and the intensities of pol I in the neighboring FC/DFC units did not correlate. 2) fluctuations with periods of 10 to 60 min, in which pol I and fibrillarin signals measured in the same unit did not correlate, but pol I signals in the units belonging to different nucleoli were synchronized. Our data indicate that a complex pulsing activity of transcription as well as early processing is common for ribosomal genes.
650    _2
$a buněčné jadérko $x chemie $x enzymologie $7 D002466
650    _2
$a chromozomální proteiny, nehistonové $x chemie $x metabolismus $7 D002868
650    _2
$a DNA řízené RNA-polymerasy $x chemie $x metabolismus $7 D012321
650    _2
$a HeLa buňky $7 D006367
650    _2
$a lidé $7 D006801
650    _2
$a imunohistochemie $7 D007150
650    _2
$a konfokální mikroskopie $7 D018613
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kováčik, L $u a Institute of Biology and Medical Genetics, First Faculty of Medicine , Charles University, and General University Hospital in Prague , Prague , Czech Republic.
700    1_
$a Mazel, T $u a Institute of Biology and Medical Genetics, First Faculty of Medicine , Charles University, and General University Hospital in Prague , Prague , Czech Republic.
700    1_
$a Cmarko, D $u a Institute of Biology and Medical Genetics, First Faculty of Medicine , Charles University, and General University Hospital in Prague , Prague , Czech Republic.
700    1_
$a Bártová, E $u a Institute of Biology and Medical Genetics, First Faculty of Medicine , Charles University, and General University Hospital in Prague , Prague , Czech Republic. b Institute of Biophysics of the CAS , Brno , Czech Republic.
700    1_
$a Raška, I $u a Institute of Biology and Medical Genetics, First Faculty of Medicine , Charles University, and General University Hospital in Prague , Prague , Czech Republic.
700    1_
$a Smirnov, E $u a Institute of Biology and Medical Genetics, First Faculty of Medicine , Charles University, and General University Hospital in Prague , Prague , Czech Republic.
773    0_
$w MED00180459 $t Nucleus (Austin, Tex.) $x 1949-1042 $g Roč. 8, č. 4 (2017), s. 421-432
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28622108 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20181008 $b ABA008
991    __
$a 20181016100105 $b ABA008
999    __
$a ok $b bmc $g 1339627 $s 1030856
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 8 $c 4 $d 421-432 $e 20170616 $i 1949-1042 $m Nucleus $n Nucleus $x MED00180459
LZP    __
$a Pubmed-20181008
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace