• Je něco špatně v tomto záznamu ?

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

H. Deschout, T. Lukes, A. Sharipov, D. Szlag, L. Feletti, W. Vandenberg, P. Dedecker, J. Hofkens, M. Leutenegger, T. Lasser, A. Radenovic,

. 2016 ; 7 (-) : 13693. [pub] 20161219

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc18034036

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI). Using simulations and fixed-cell focal adhesion images, we investigate the complementarity between PALM and SOFI in terms of spatial and temporal resolution. This PALM-SOFI framework is used to image focal adhesions in living cells, while obtaining a temporal resolution below 10 s. We visualize the dynamics of focal adhesions, and reveal local mean velocities around 190 nm min-1. The complementarity of PALM and SOFI is assessed in detail with a methodology that integrates a resolution and signal-to-noise metric. This PALM and SOFI concept provides an enlarged quantitative imaging framework, allowing unprecedented functional exploration of focal adhesions through the estimation of molecular parameters such as fluorophore densities and photoactivation or photoswitching kinetics.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18034036
003      
CZ-PrNML
005      
20181009110328.0
007      
ta
008      
181008s2016 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/ncomms13693 $2 doi
035    __
$a (PubMed)27991512
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Deschout, Hendrik $u Laboratory of Nanoscale Biology &Laboratoire d'Optique Biomédicale, STI - IBI, Ecole Polytechnique Fédérale de Lausanne, Station 17, CH-1015 Lausanne, Switzerland.
245    10
$a Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions / $c H. Deschout, T. Lukes, A. Sharipov, D. Szlag, L. Feletti, W. Vandenberg, P. Dedecker, J. Hofkens, M. Leutenegger, T. Lasser, A. Radenovic,
520    9_
$a Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI). Using simulations and fixed-cell focal adhesion images, we investigate the complementarity between PALM and SOFI in terms of spatial and temporal resolution. This PALM-SOFI framework is used to image focal adhesions in living cells, while obtaining a temporal resolution below 10 s. We visualize the dynamics of focal adhesions, and reveal local mean velocities around 190 nm min-1. The complementarity of PALM and SOFI is assessed in detail with a methodology that integrates a resolution and signal-to-noise metric. This PALM and SOFI concept provides an enlarged quantitative imaging framework, allowing unprecedented functional exploration of focal adhesions through the estimation of molecular parameters such as fluorophore densities and photoactivation or photoswitching kinetics.
650    _2
$a zvířata $7 D000818
650    _2
$a buněčná adheze $x fyziologie $7 D002448
650    _2
$a fibroblasty $x fyziologie $7 D005347
650    _2
$a myši $7 D051379
650    _2
$a mikroskopie $x metody $7 D008853
650    _2
$a paxilin $x chemie $x genetika $x metabolismus $7 D051419
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a barvení a značení $7 D013194
650    _2
$a časové faktory $7 D013997
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Lukes, Tomas $u Laboratory of Nanoscale Biology &Laboratoire d'Optique Biomédicale, STI - IBI, Ecole Polytechnique Fédérale de Lausanne, Station 17, CH-1015 Lausanne, Switzerland. Department of Radioelectronics, FEE, Czech Technical University in Prague, Technická 2, 166 27 Prague 6, Czech Republic.
700    1_
$a Sharipov, Azat $u Laboratory of Nanoscale Biology &Laboratoire d'Optique Biomédicale, STI - IBI, Ecole Polytechnique Fédérale de Lausanne, Station 17, CH-1015 Lausanne, Switzerland.
700    1_
$a Szlag, Daniel $u Laboratory of Nanoscale Biology &Laboratoire d'Optique Biomédicale, STI - IBI, Ecole Polytechnique Fédérale de Lausanne, Station 17, CH-1015 Lausanne, Switzerland.
700    1_
$a Feletti, Lely $u Laboratory of Nanoscale Biology &Laboratoire d'Optique Biomédicale, STI - IBI, Ecole Polytechnique Fédérale de Lausanne, Station 17, CH-1015 Lausanne, Switzerland.
700    1_
$a Vandenberg, Wim $u Department of Chemistry, University of Leuven, Celestijnenlaan 200F, B-3001 Heverlee, Belgium.
700    1_
$a Dedecker, Peter $u Department of Chemistry, University of Leuven, Celestijnenlaan 200F, B-3001 Heverlee, Belgium.
700    1_
$a Hofkens, Johan $u Department of Chemistry, University of Leuven, Celestijnenlaan 200F, B-3001 Heverlee, Belgium.
700    1_
$a Leutenegger, Marcel $u Abteilung NanoBiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077 Göttingen, Germany.
700    1_
$a Lasser, Theo $u Laboratory of Nanoscale Biology &Laboratoire d'Optique Biomédicale, STI - IBI, Ecole Polytechnique Fédérale de Lausanne, Station 17, CH-1015 Lausanne, Switzerland.
700    1_
$a Radenovic, Aleksandra $u Laboratory of Nanoscale Biology &Laboratoire d'Optique Biomédicale, STI - IBI, Ecole Polytechnique Fédérale de Lausanne, Station 17, CH-1015 Lausanne, Switzerland.
773    0_
$w MED00184850 $t Nature communications $x 2041-1723 $g Roč. 7, č. - (2016), s. 13693
856    41
$u https://pubmed.ncbi.nlm.nih.gov/27991512 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20181008 $b ABA008
991    __
$a 20181009110816 $b ABA008
999    __
$a ok $b bmc $g 1340406 $s 1031030
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 7 $c - $d 13693 $e 20161219 $i 2041-1723 $m Nature communications $n Nat Commun $x MED00184850
LZP    __
$a Pubmed-20181008

Najít záznam

Citační ukazatele

Možnosti archivace