-
Je něco špatně v tomto záznamu ?
NTPDase, 5′-nucleotidase and adenosine deaminase activities and purine levels in serum of sickle cell anemia patients
Lívia G. Castilhos, Stephen A. Adefegha, Pedro H. Doleski, Tatiana M.D. Bertoldo, Cesar Eduardo J. Moritz, Emerson A. Casali, Daniela B.R. Leal
Jazyk angličtina Země Česko
Typ dokumentu práce podpořená grantem
- Klíčová slova
- NTPDáza, enzymatická aktivita,
- MeSH
- 5'-nukleotidasa krev MeSH
- adenosindeaminasa krev MeSH
- dospělí MeSH
- hydroxymočovina terapeutické užití MeSH
- kyselina listová terapeutické užití MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- nukleosidy krev MeSH
- nukleotidy krev MeSH
- puriny krev MeSH
- srpkovitá anemie * enzymologie patologie MeSH
- techniky in vitro MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
Sickle cell anemia (SCA) is a hereditary disorder that is characterized by tendency of hemoglobin molecules within the erythrocytes to polymerize under hypoxia conditions, deform the red cells, and promote vaso-occlusion and endothelial damage. This disease may be associated with the extracellular release of nucleotides, particularly ATP, ADP and adenosine into the circulation. The aim of this study was to investigate the possible changes in adenine nucleotides and nucleoside metabolizing enzymes as well as their levels in serum of SCA patients. NTPDase, 5′-nucleotidase and adenosine deaminase activities were evaluated in serum obtained from blood samples of 15 SCA treated patients and 15 healthy subjects (control group). The results revealed that there were significant (P < 0.001) elevations in NTPDase, 5′-nucleotidase and ADA activities in serum of SCA treated patients when compared to the control group. Furthermore, no significant (P > 0.05) alteration was observed in ATP, ADP and adenosine levels of both SCA treated and control groups. However, inosine level was significantly (P < 0.05) decreased and hypoxanthine level was higher in SCA treated patients (P < 0.001) when compared to the control group. The results suggest the involvement of purinergic signaling enzymes in the maintenance of the levels of extracellular nucleotides and nucleosides, thus preventing some pathophysiological conditions associated with SCA.
Citace poskytuje Crossref.org
Literatura
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18039974
- 003
- CZ-PrNML
- 005
- 20190304162403.0
- 007
- ta
- 008
- 181211s2018 xr d f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.jab.2017.12.004 $2 doi
- 040 __
- $a ABA008 $d ABA008 $e AACR2 $b cze
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Castilhos, Lívia G. $u Federal University of Santa Maria, Post-Graduate Program in Pharmaceutical Sciences, Santa Maria, RS, Brazil
- 245 10
- $a NTPDase, 5′-nucleotidase and adenosine deaminase activities and purine levels in serum of sickle cell anemia patients / $c Lívia G. Castilhos, Stephen A. Adefegha, Pedro H. Doleski, Tatiana M.D. Bertoldo, Cesar Eduardo J. Moritz, Emerson A. Casali, Daniela B.R. Leal
- 504 __
- $a Literatura
- 520 9_
- $a Sickle cell anemia (SCA) is a hereditary disorder that is characterized by tendency of hemoglobin molecules within the erythrocytes to polymerize under hypoxia conditions, deform the red cells, and promote vaso-occlusion and endothelial damage. This disease may be associated with the extracellular release of nucleotides, particularly ATP, ADP and adenosine into the circulation. The aim of this study was to investigate the possible changes in adenine nucleotides and nucleoside metabolizing enzymes as well as their levels in serum of SCA patients. NTPDase, 5′-nucleotidase and adenosine deaminase activities were evaluated in serum obtained from blood samples of 15 SCA treated patients and 15 healthy subjects (control group). The results revealed that there were significant (P < 0.001) elevations in NTPDase, 5′-nucleotidase and ADA activities in serum of SCA treated patients when compared to the control group. Furthermore, no significant (P > 0.05) alteration was observed in ATP, ADP and adenosine levels of both SCA treated and control groups. However, inosine level was significantly (P < 0.05) decreased and hypoxanthine level was higher in SCA treated patients (P < 0.001) when compared to the control group. The results suggest the involvement of purinergic signaling enzymes in the maintenance of the levels of extracellular nucleotides and nucleosides, thus preventing some pathophysiological conditions associated with SCA.
- 650 12
- $a srpkovitá anemie $x enzymologie $x patologie $7 D000755
- 650 _2
- $a adenosindeaminasa $x krev $7 D000243
- 650 _2
- $a 5'-nukleotidasa $x krev $7 D015720
- 650 _2
- $a nukleosidy $x krev $7 D009705
- 650 _2
- $a puriny $x krev $7 D011687
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a mladý dospělý $7 D055815
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a kyselina listová $x terapeutické užití $7 D005492
- 650 _2
- $a hydroxymočovina $x terapeutické užití $7 D006918
- 650 _2
- $a techniky in vitro $7 D066298
- 650 _2
- $a nukleotidy $x krev $7 D009711
- 653 00
- $a NTPDáza
- 653 00
- $a enzymatická aktivita
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Adefegha, Stephen A. $u Federal University of Santa Maria, Post-Graduate Program in Toxicological Biochemistry, Santa Maria, RS, Brazil; Federal University of Technology, Department of Biochemistry, Akure, Nigeria
- 700 1_
- $a Doleski, Pedro H. $u Federal University of Santa Maria, Post-Graduate Program in Toxicological Biochemistry, Santa Maria, RS, Brazil
- 700 1_
- $a Bertoldo, Tatiana M.D. $u Federal University of Santa Maria, Post-Graduate Program in Pharmaceutical Sciences, Santa Maria, RS, Brazil
- 700 1_
- $a Moritz, Cesar Eduardo J. $u Federal University of Rio Grande do Sul, Post-Graduate Program in Science of Human Movement, Porto Alegre, RS, Brazil
- 700 1_
- $a Casali, Emerson A. $u Federal University of Rio Grande do Sul, Post-Graduate Program in Science of Human Movement, Porto Alegre, RS, Brazil
- 700 1_
- $a Leal, Daniela B.R. $u Federal University of Santa Maria, Post-Graduate Program in Pharmaceutical Sciences, Santa Maria, RS, Brazil; Federal University of Santa Maria, Post-Graduate Program in Toxicological Biochemistry, Santa Maria, RS, Brazil
- 773 0_
- $t Journal of applied biomedicine $x 1214-021X $g Roč. 16, č. 3 (2018), s. 208-213 $w MED00012667
- 856 41
- $u https://jab.zsf.jcu.cz/pdfs/jab/2018/03/08.pdf $y plný text volně přístupný
- 910 __
- $a ABA008 $b B 2301 $c 1249 $y 4 $z 0
- 990 __
- $a 20181211090250 $b ABA008
- 991 __
- $a 20190304162715 $b ABA008
- 999 __
- $a ok $b bmc $g 1358048 $s 1037037
- BAS __
- $a 3
- BMC __
- $a 2018 $b 16 $c 3 $d 208-213 $i 1214-021X $m Journal of Applied Biomedicine $x MED00012667
- LZP __
- $c NLK185 $d 20190304 $a NLK 2018-52/dk
