-
Something wrong with this record ?
Comparison of cation-exchange capillary columns used for ion chromatographic separation of biogenic amines
Y. Li, PN. Nesterenko, R. Stanley, B. Paull, M. Macka,
Language English Country Netherlands
Document type Journal Article
- MeSH
- Amino Acids chemistry MeSH
- Biogenic Amines analysis isolation & purification MeSH
- Chromatography, Ion Exchange methods MeSH
- Cations chemistry MeSH
- Hydrogen-Ion Concentration MeSH
- Carboxylic Acids chemistry MeSH
- Hydrogen Bonding MeSH
- Publication type
- Journal Article MeSH
The selectivity for 15 biogenic amines and amino acids shown by three capillary cation-exchange columns, IonPac CS19, CS12A and CS17 (250 × 0.4 mm ID, all from Thermo Fisher Scientific), exhibiting medium, medium low and ultra-low hydrophobicity, and either carboxylic or mixed carboxylic/phosphonic acid functional groups, was investigated. A mixed mode retention mechanism was revealed with ion-exchange, hydrophobic and hydrogen bonding interactions contributing to retention of polar organic molecules on these phases. The relative impact of these interactions was evaluated via the effect of concentration and pH of the eluent (methanesulfonic acid) on the retention of fifteen structurally similar biogenic amines and amino acids. Strong hydrogen bonding interactions were observed between the solute amino acid carboxylic groups and cation-exchange groups from the ion-exchangers. This is the first time retention data correlated with logP data has revealed clustering of the solutes in two groups, according to the presence or absence of a carboxylic acid functional group. In addition, stronger retention behaviour was found for the IonPac CS12A cation-exchanger, containing both carboxylic and phosphonic functional groups. Further assessment of the orthogonality plots of retention factors for the three stationary phases revealed that the columns exhibited different complimentary selectivity that can be utilised to achieve specific separations.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19000418
- 003
- CZ-PrNML
- 005
- 20190108125657.0
- 007
- ta
- 008
- 190107s2018 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.chroma.2018.08.021 $2 doi
- 035 __
- $a (PubMed)30150118
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Li, Yan $u School of Natural Sciences and Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Hobart 7001, Australia.
- 245 10
- $a Comparison of cation-exchange capillary columns used for ion chromatographic separation of biogenic amines / $c Y. Li, PN. Nesterenko, R. Stanley, B. Paull, M. Macka,
- 520 9_
- $a The selectivity for 15 biogenic amines and amino acids shown by three capillary cation-exchange columns, IonPac CS19, CS12A and CS17 (250 × 0.4 mm ID, all from Thermo Fisher Scientific), exhibiting medium, medium low and ultra-low hydrophobicity, and either carboxylic or mixed carboxylic/phosphonic acid functional groups, was investigated. A mixed mode retention mechanism was revealed with ion-exchange, hydrophobic and hydrogen bonding interactions contributing to retention of polar organic molecules on these phases. The relative impact of these interactions was evaluated via the effect of concentration and pH of the eluent (methanesulfonic acid) on the retention of fifteen structurally similar biogenic amines and amino acids. Strong hydrogen bonding interactions were observed between the solute amino acid carboxylic groups and cation-exchange groups from the ion-exchangers. This is the first time retention data correlated with logP data has revealed clustering of the solutes in two groups, according to the presence or absence of a carboxylic acid functional group. In addition, stronger retention behaviour was found for the IonPac CS12A cation-exchanger, containing both carboxylic and phosphonic functional groups. Further assessment of the orthogonality plots of retention factors for the three stationary phases revealed that the columns exhibited different complimentary selectivity that can be utilised to achieve specific separations.
- 650 _2
- $a aminokyseliny $x chemie $7 D000596
- 650 _2
- $a biogenní aminy $x analýza $x izolace a purifikace $7 D001679
- 650 _2
- $a kyseliny karboxylové $x chemie $7 D002264
- 650 _2
- $a kationty $x chemie $7 D002412
- 650 _2
- $a chromatografie iontoměničová $x metody $7 D002852
- 650 _2
- $a vodíková vazba $7 D006860
- 650 _2
- $a koncentrace vodíkových iontů $7 D006863
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Nesterenko, Pavel N $u School of Natural Sciences and Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Hobart 7001, Australia; ARC Training Centre for Portable Analytical Separation Technologies (ASTech), University of Tasmania, Private Bag 75, Hobart 7001, Australia.
- 700 1_
- $a Stanley, Roger $u Centre for Food Innovation, University of Tasmania, Locked Bag 1370, Launceston 7250, Australia.
- 700 1_
- $a Paull, Brett $u School of Natural Sciences and Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Hobart 7001, Australia; ARC Training Centre for Portable Analytical Separation Technologies (ASTech), University of Tasmania, Private Bag 75, Hobart 7001, Australia.
- 700 1_
- $a Macka, Mirek $u School of Natural Sciences and Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Hobart 7001, Australia; Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic. Electronic address: Mirek.Macka@utas.edu.au.
- 773 0_
- $w MED00004962 $t Journal of chromatography. A $x 1873-3778 $g Roč. 1571, č. - (2018), s. 193-200
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/30150118 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190107 $b ABA008
- 991 __
- $a 20190108125858 $b ABA008
- 999 __
- $a ok $b bmc $g 1364504 $s 1038541
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 1571 $c - $d 193-200 $e 20180810 $i 1873-3778 $m Journal of chromatography. A, Including electrophoresis and other separation methods $n J Chromatogr A $x MED00004962
- LZP __
- $a Pubmed-20190107
