Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.

Agricultural Institute of Slovenia Hacquetova ulica 17 1000 Ljubljana Slovenia

Department of Plant Pathology Division of Agriculture University of Arkansas System Fayetteville AR 72701 USA

Department of Plant Virology Institute of Plant Molecular Biology Biology Centre of the Academy of Sciences of the Czech Republic v v i Branišovská 31 370 05 České Budějovice Czech Republic

National Clonal Germplasm Repository United States Department of Agriculture Corvallis OR 97333 USA

Plant Pathology Laboratory TERRA Gembloux Agro Bio Tech University of Liège Passage des Déportés 2 5030 Gembloux Belgium

Virology Phytoplasmology Laboratory Agroscope 1260 Nyon Switzerland

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$a Koloniuk, Igor $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic. koloniuk@umbr.cas.cz.

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$a Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species / $c I. Koloniuk, T. Thekke-Veetil, JS. Reynard, I. Mavrič Pleško, J. Přibylová, J. Brodard, I. Kellenberger, T. Sarkisova, J. Špak, J. Lamovšek, S. Massart, T. Ho, JD. Postman, IE. Tzanetakis,

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$a Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.

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$a Thekke-Veetil, Thanuja $u Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, USA. thanujatv@gmail.com.

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$a Reynard, Jean-Sébastien $u Virology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, Switzerland. jean-sebastien.reynard@agroscope.admin.ch.

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$a Mavrič Pleško, Irena $u Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000 Ljubljana, Slovenia. irena.mavricplesko@kis.si.

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$a Přibylová, Jaroslava $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic. pribyl@umbr.cas.cz.

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$a Brodard, Justine $u Virology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, Switzerland. justine.brodard@agroscope.admin.ch.

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$a Kellenberger, Isabelle $u Virology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, Switzerland. isabelle.kellenberger@agroscope.admin.ch.

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$a Sarkisova, Tatiana $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic. sarkisova@umbr.cas.cz.

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$a Špak, Josef $u Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic. spak@umbr.cas.cz.

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$a Lamovšek, Janja $u Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000 Ljubljana, Slovenia. janja.lamovsek@kis.si.

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$a Massart, Sebastien $u Plant Pathology Laboratory, TERRA-Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés, 2, 5030 Gembloux, Belgium. sebastien.massart@uliege.be.

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$a Ho, Thien $u Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, USA. txho@uark.edu.

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$a Postman, Joseph D $u National Clonal Germplasm Repository, United States Department of Agriculture, Corvallis, OR 97333, USA. joseph.postman@ars.usda.gov.

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$a Tzanetakis, Ioannis E $u Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, USA. itzaneta@uark.edu.

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