Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.

• MeSH
• Closterovirus klasifikace   genetika   izolace a purifikace   ultrastruktura   MeSH
• fylogeneze MeSH
• genetická variace MeSH
• genom virový MeSH
• genomika metody   MeSH
• nemoci rostlin virologie   MeSH
• otevřené čtecí rámce MeSH
• rekombinace genetická MeSH
• Ribes virologie   MeSH
• RNA virová MeSH
• sekvence aminokyselin MeSH
• vysoce účinné nukleotidové sekvenování MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH

The complete nucleotide sequences of RNA1 and RNA2 of the Holandský červený strain of currant latent virus (CuLV) were determined using next-generation sequencing. The RNA1 is predicted to encode a polyprotein 2124 amino acid long with RdRp motifs. The RNA2 is predicted to encode a polyprotein 957 amino acid long with homology to the capsid protein of apple latent spherical virus and cherry rasp leaf virus. Phylogenetic analysis confirms that CuLV is a new distinct member of the genus Cheravirus.

• MeSH
• fylogeneze MeSH
• genom virový * MeSH
• molekulární sekvence - údaje MeSH
• Ribes virologie   MeSH
• RNA virová genetika   MeSH
• RNA-viry genetika   izolace a purifikace   MeSH
• rostlinné viry genetika   izolace a purifikace   MeSH
• sekvenční analýza DNA MeSH
• sekvenční homologie MeSH
• shluková analýza MeSH
• vysoce účinné nukleotidové sekvenování MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The complete nucleotide sequence of a novel virus from red currant, provisionally named currant virus A (CuVA), was determined. The genome is 7925 nucleotides long and has a 3'-poly(A) tail. The genome organization with two overlapping open reading frames is similar to that of capilloviruses, but the CuVA genome is about 600 nucleotides longer than that of the longest known capillovirus, cherry virus A. The RNA is predicted to encode a polyprotein with domains of methyltransferase, 2OG-Fe(II) oxygenase, papain-like protease, RNA helicase, RdRp, and capsid protein. Phylogenetic analysis confirms that CuVA is a new and distinct member of the genus Capillovirus.

• MeSH
• Flexiviridae genetika   izolace a purifikace   MeSH
• fylogeneze MeSH
• genom virový MeSH
• nemoci rostlin virologie   MeSH
• regulace exprese virových genů fyziologie   MeSH
• Ribes virologie   MeSH
• RNA virová MeSH
• virové proteiny genetika   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH