The complete nucleotide sequence of a novel virus from red currant, provisionally named currant virus A (CuVA), was determined. The genome is 7925 nucleotides long and has a 3'-poly(A) tail. The genome organization with two overlapping open reading frames is similar to that of capilloviruses, but the CuVA genome is about 600 nucleotides longer than that of the longest known capillovirus, cherry virus A. The RNA is predicted to encode a polyprotein with domains of methyltransferase, 2OG-Fe(II) oxygenase, papain-like protease, RNA helicase, RdRp, and capsid protein. Phylogenetic analysis confirms that CuVA is a new and distinct member of the genus Capillovirus.
- MeSH
- Flexiviridae genetika izolace a purifikace MeSH
- fylogeneze MeSH
- genom virový MeSH
- nemoci rostlin virologie MeSH
- regulace exprese virových genů fyziologie MeSH
- Ribes virologie MeSH
- RNA virová MeSH
- virové proteiny genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A novel tritimovirus of the family Potyviridae was isolated from tall oatgrass, Arrhenatherum elatius, exhibiting mosaic symptoms. The virus, for which the name tall oatgrass mosaic virus (TOgMV) is coined, has a filamentous particle of 720 nm and is associated with pinwheel inclusion bodies characteristic of members of the family Potyviridae. The virus was mechanically transmitted to tall oatgrass seedlings, which subsequently exhibited mosaic symptoms. The experimental host range was limited to a few monocot species. The complete genome sequence of TOgMV was determined to be 9359 nucleotides, excluding the 3' polyadenylated tail. The viral RNA encodes one large putative open reading frame of 3029 amino acids with a genome organization typical of monopartite potyvirids. Pairwise comparison of putative mature proteins and proteinase cleavage sites indicated that TOgMV is most closely related to members of the genus Tritimovirus. Phylogenetic analysis of the complete polyprotein and CP sequences of representative members of the family Potyviridae indicate that TOgMV is a distinct tritimovirus naturally infecting tall oatgrass.
- MeSH
- druhová specificita MeSH
- fylogeneze MeSH
- lipnicovité virologie MeSH
- listy rostlin virologie MeSH
- nemoci rostlin virologie MeSH
- Potyviridae klasifikace genetika izolace a purifikace fyziologie MeSH
- regulace exprese virových genů fyziologie MeSH
- virové proteiny genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
The aim of this work was to express recombinant nonstructural Nsp7 protein of European genotype of porcine reproductive and respiratory syndrome virus and to evaluate its diagnostic sensitivity and specificity in serological diagnostics of the disease. The gene coding for Nsp7 protein was expressed in Escherichia coli cells and purified by IMAC. Serological reactivity of purified protein was assessed on a panel of swine sera in indirect ELISA test. Serum samples originated from PRRS positive farms, herds free of PRRS infection and PRRS free herds vaccinated with an inactivated vaccine. Nsp7 antigen proved to be suitable for serological detection of PRRS specific antibodies, showing diagnostic sensitivity of 82.2% and specificity of 97.6% when compared with IDEXX ELISA test. The nonstructural protein proved to be suitable for use as an antigen for the differentiation of post-infection and post-vaccination antibodies in pigs vaccinated with the inactivated vaccine. But the low overall antibody response to N protein after this type of vaccination makes this concept rather theoretical.
- MeSH
- antigeny virové MeSH
- ELISA MeSH
- Escherichia coli MeSH
- genotyp MeSH
- protilátky virové MeSH
- regulace exprese virových genů fyziologie MeSH
- sérologické testy MeSH
- specificita protilátek MeSH
- virové proteiny genetika metabolismus MeSH
- virus reprodukčního a respiračního syndromu prasat genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Retroviry představují viry s unikátním způsobem replikace a zároveň jsou původcem mnoha lidských i zvířecích onemocnění, na prvním místě AIDS způsobovaný retrovirem HIV. Článek shrnuje základy současného taxonomického rozdělení retrovirů a popisuje životní cyklus retrovirů.
Retroviruses are viruses with unique way of replication and they are the cause of many human and animal diseases. The most prominent example is AIDS caused by HIV retrovirus. The article summarizes the taxonomy of the retroviruses and describes retroviral life cycle.
