Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.
- MeSH
- Closterovirus klasifikace genetika izolace a purifikace ultrastruktura MeSH
- fylogeneze MeSH
- genetická variace MeSH
- genom virový MeSH
- genomika metody MeSH
- nemoci rostlin virologie MeSH
- otevřené čtecí rámce MeSH
- rekombinace genetická MeSH
- Ribes virologie MeSH
- RNA virová MeSH
- sekvence aminokyselin MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
