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GHEP-ISFG collaborative exercise on mixture profiles (GHEP-MIX06). Reporting conclusions: Results and evaluation
PA. Barrio, M. Crespillo, JA. Luque, M. Aler, C. Baeza-Richer, L. Baldassarri, E. Carnevali, P. Coufalova, I. Flores, O. García, MA. García, R. González, A. Hernández, V. Inglés, GM. Luque, A. Mosquera-Miguel, S. Pedrosa, ML. Pontes, MJ. Porto,...
Language English Country Netherlands
Document type Journal Article
- MeSH
- DNA Fingerprinting standards MeSH
- Laboratories statistics & numerical data MeSH
- Humans MeSH
- Microsatellite Repeats * MeSH
- Likelihood Functions MeSH
- Software MeSH
- Forensic Genetics standards MeSH
- Societies, Scientific * MeSH
- Research Report standards MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
One of the main goals of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the field of forensic genetics. Due to this fact, GHEP-ISFG holds different working commissions that are set up to develop activities in scientific aspects of general interest. One of them, the Mixture Commission of GHEP-ISFG, has organized annually, since 2009, a collaborative exercise on analysis and interpretation of autosomal short tandem repeat (STR) mixture profiles. Until now, six exercises have been organized. At the present edition (GHEP-MIX06), with 25 participant laboratories, the exercise main aim was to assess mixture profiles results by issuing a report, from the proposal of a complex mock case. One of the conclusions obtained from this exercise is the increasing tendency of participating laboratories to validate DNA mixture profiles analysis following international recommendations. However, the results have shown some differences among them regarding the edition and also the interpretation of mixture profiles. Besides, although the last revision of ISO/IEC 17025:2017 gives indications of how results should be reported, not all laboratories strictly follow their recommendations. Regarding the statistical aspect, all those laboratories that have performed statistical evaluation of the data have employed the likelihood ratio (LR) as a parameter to evaluate the statistical compatibility. However, LR values obtained show a wide range of variation. This fact could not be attributed to the software employed, since the vast majority of laboratories that performed LR calculation employed the same software (LRmixStudio). Thus, the final allelic composition of the edited mixture profile and the parameters employed in the software could explain this data dispersion. This highlights the need, for each laboratory, to define through internal validations its criteria for editing and interpreting mixtures, and to continuous train in software handling.
Área de Laboratorio NASERTIC Navarra Spain
Departamento de Biología Servicio de Criminalística de la Guardia Civil Madrid Spain
Forensic Science Unit Forensic Genetics Section Basque Country Police Erandio Bizkaia Spain
Grupo Investigación Identificación Genética IdentiGEN Universidad de Antioquia Medellín Colombia
Institute of Criminalistics Prague Prague Czech Republic
Instituto de Medicina Legal y Ciencias Forenses de Valencia Spain
INTCF National Institute of Toxicology and Forensic Science Department of Barcelona Spain
INTCF National Institute of Toxicology and Forensic Science Department of Madrid Spain
INTCF National Institute of Toxicology and Forensic Science Department of Sevilla Spain
Laboratorio Regional de Genética Forense Poder Judicial de Rio Negro Argentina
Mixture Commission of the GHEP ISFG Spain
Registro Nacional ADN Servicio Médico Legal Santiago de Chile Chile
Serviço de Genética e Biologia Forenses INMLCF IP Delegação do Nort Porto Portugal
Unidad de Genética Forense Servicio Médico Legal de Santiago Chile
References provided by Crossref.org
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