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Recent progress in nucleic acids isotachophoresis
V. Datinská, I. Voráčová, U. Schlecht, J. Berka, F. Foret,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, přehledy
PubMed
28980403
DOI
10.1002/jssc.201700878
Knihovny.cz E-zdroje
- MeSH
- automatizace MeSH
- DNA analýza MeSH
- elektrolyty MeSH
- hybridizace nukleových kyselin MeSH
- izotachoforéza metody trendy MeSH
- lidé MeSH
- mikro RNA analýza MeSH
- mikrofluidika MeSH
- nukleové kyseliny izolace a purifikace MeSH
- polymerázová řetězová reakce MeSH
- pufry MeSH
- tělesné tekutiny metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Progress achieved between 2014-2017 in the extraction and sample preparation of nucleic acid by isotachophoresis is reviewed in this paper. The isolation and purification of nucleic acids is very often compromised by a complex matrix such as blood and other bodily fluids, samples from the scene of crime, fossil samples, etc. While most of the common nucleic acids isolation techniques are based on extraction with inherent limitations with regard to quantitative results, isotachophoretic focusing is a quantitative process with a theoretically unlimited concentration factor. Since isotachophoresis belongs to less traditional approaches of nucleic acids purification, we present not only the latest developments in the application of isotachophoresis for the nucleic acids concentration but also a brief description of the principles of this method.
Citace poskytuje Crossref.org
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- $a Progress achieved between 2014-2017 in the extraction and sample preparation of nucleic acid by isotachophoresis is reviewed in this paper. The isolation and purification of nucleic acids is very often compromised by a complex matrix such as blood and other bodily fluids, samples from the scene of crime, fossil samples, etc. While most of the common nucleic acids isolation techniques are based on extraction with inherent limitations with regard to quantitative results, isotachophoretic focusing is a quantitative process with a theoretically unlimited concentration factor. Since isotachophoresis belongs to less traditional approaches of nucleic acids purification, we present not only the latest developments in the application of isotachophoresis for the nucleic acids concentration but also a brief description of the principles of this method.
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