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Biocompatibility of the human mesenchymal stem cells with bovine bone tissue at the cellular level in vitro
Marianna Trebunova, Sylvia Gromošová, Darina Bačenková, Ján Rosocha, Jozef Živčák
Language English Country Czech Republic
- MeSH
- Biocompatible Materials * MeSH
- Cell Culture Techniques MeSH
- Microscopy, Electron MeSH
- Bone and Bones MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Mesenchymal Stem Cells * MeSH
- Flow Cytometry MeSH
- Materials Testing MeSH
- Tissue Extracts MeSH
- Check Tag
- Humans MeSH
The purpose of this study was to investigate biocompatibility of the human mesenchymal stem cells with bovine bone tissue at the cellular level in vitro. Phenotypic analysis of cells was made by flow cytometry. Cells were grown on the bone for 12 days. Metabolic activity of cells was assessed with the MTS assay. The growth data were used to calculate the population doubling times. The scanning electron microscopy was used to verify the attachment of cells on the bone surface. The results were analyzed by using ANOVA test. Immunophenotypic characteristics were positive for CD105, CD90, CD73, and negative for CD34, CD45. The growth curves of stem cells of the 1st and the 2nd passages for both media, with and without, bovine bone were constructed. The increase of approximately 60% of the doubling time for mesenchymal cells co-cultivated with bovine bone tissue was observed for both passages in comparison with the control.Our study confirmed that mesenchymal stem cells are able to adhere to the bovine bone, even not being modified with bone-targeting elements. The proliferation rate and metabolic activity of cells co-cultivated with bone decrease in comparison with the control. Better survival was observed for cells of the 1st passage.
Associated Tissue Bank Faculty of Medicine Pavol Jozef Šafárik University Košice
Associated Tissue Bank of University Hospital of L Pasteur Košice
Department of Biomedical Engineering and Measurement Technical University Košice
Literatura
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- $a The purpose of this study was to investigate biocompatibility of the human mesenchymal stem cells with bovine bone tissue at the cellular level in vitro. Phenotypic analysis of cells was made by flow cytometry. Cells were grown on the bone for 12 days. Metabolic activity of cells was assessed with the MTS assay. The growth data were used to calculate the population doubling times. The scanning electron microscopy was used to verify the attachment of cells on the bone surface. The results were analyzed by using ANOVA test. Immunophenotypic characteristics were positive for CD105, CD90, CD73, and negative for CD34, CD45. The growth curves of stem cells of the 1st and the 2nd passages for both media, with and without, bovine bone were constructed. The increase of approximately 60% of the doubling time for mesenchymal cells co-cultivated with bovine bone tissue was observed for both passages in comparison with the control.Our study confirmed that mesenchymal stem cells are able to adhere to the bovine bone, even not being modified with bone-targeting elements. The proliferation rate and metabolic activity of cells co-cultivated with bone decrease in comparison with the control. Better survival was observed for cells of the 1st passage.
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