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Green tea can supress rabbit ovarian functions in vitro and in vivo
A. Baláži, AV. Sirotkin, M. Földešiová, P. Makovický, Ľ. Chrastinová, P. Makovický, P. Chrenek,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, přehledy
- MeSH
- apoptóza MeSH
- čaj chemie MeSH
- fertilita účinky léků MeSH
- kaspasa 3 metabolismus MeSH
- katechin analogy a deriváty izolace a purifikace farmakologie MeSH
- králíci * MeSH
- ovarium účinky léků metabolismus MeSH
- polyfenoly izolace a purifikace farmakologie MeSH
- resveratrol izolace a purifikace farmakologie MeSH
- zvířata MeSH
- Check Tag
- králíci * MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
The aim of present study was to evaluate the action of green tea and its constituents on rabbit ovarian functions and some non-reproductive indexes. In in vitro experiments, rabbit ovarian fragments were cultured with green tea constituents - epigallocatechin-3-gallate (EGCG), green tea polyphenols (GTPP) and resveratrol (RSV) (at 0, 1, 10 or 100 μg/mL medium). The accumulation of an apoptosis marker - caspase 3 and the release of progesterone (P4) and testosterone (T) were measured. In in vivo experiments, does were fed a standard diet or a diet enriched with green tea powder. The weight gain, mortality, ovarian length and weight, conception and kindling rate, number of liveborn, stillborn, and weaned pups, diameter of ovarian follicles and some blood haematological and biochemical parameters were analysed. Culture of ovarian fragments with EGCG increased accumulation of caspase 3, whilst both GTTP and RSV decreased it. EGCG inhibited both P4 and T output, GTPP stimulated P4 and inhibited T, whilst RSV promoted release of both P4 and T. Feeding with green tea increased ovarian length and diameter of ovarian non-ovulated peri-ovulatory haemorrhagic but not of primary and secondary growing follicles. Furthermore, green tea reduced conception and kindling rate, the number of liveborn and weaned pups, increased female mortality but not their weight gain. It reduced platelet distribution width, but it did not affect other haematological and biochemical indexes. These observations suggest that dietary green tea can reduce rabbit doe's viability, ovarian functions and fecundity, perhaps due to changes in ovarian cell apoptosis, steroid hormones release and blockade of the ovulation of large ovarian follicles. The anti-reproductive action of green tea could be due to its constituent - EGCG with pro-apoptotic and anti-steroid hormone properties.
Citace poskytuje Crossref.org
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- $a Baláži, Andrej $u National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Lužianky, Slovak Republic. Electronic address: balazi.andrej@gmail.com.
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- $a The aim of present study was to evaluate the action of green tea and its constituents on rabbit ovarian functions and some non-reproductive indexes. In in vitro experiments, rabbit ovarian fragments were cultured with green tea constituents - epigallocatechin-3-gallate (EGCG), green tea polyphenols (GTPP) and resveratrol (RSV) (at 0, 1, 10 or 100 μg/mL medium). The accumulation of an apoptosis marker - caspase 3 and the release of progesterone (P4) and testosterone (T) were measured. In in vivo experiments, does were fed a standard diet or a diet enriched with green tea powder. The weight gain, mortality, ovarian length and weight, conception and kindling rate, number of liveborn, stillborn, and weaned pups, diameter of ovarian follicles and some blood haematological and biochemical parameters were analysed. Culture of ovarian fragments with EGCG increased accumulation of caspase 3, whilst both GTTP and RSV decreased it. EGCG inhibited both P4 and T output, GTPP stimulated P4 and inhibited T, whilst RSV promoted release of both P4 and T. Feeding with green tea increased ovarian length and diameter of ovarian non-ovulated peri-ovulatory haemorrhagic but not of primary and secondary growing follicles. Furthermore, green tea reduced conception and kindling rate, the number of liveborn and weaned pups, increased female mortality but not their weight gain. It reduced platelet distribution width, but it did not affect other haematological and biochemical indexes. These observations suggest that dietary green tea can reduce rabbit doe's viability, ovarian functions and fecundity, perhaps due to changes in ovarian cell apoptosis, steroid hormones release and blockade of the ovulation of large ovarian follicles. The anti-reproductive action of green tea could be due to its constituent - EGCG with pro-apoptotic and anti-steroid hormone properties.
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- $a Sirotkin, Alexander V $u Constantine the Philosopher University, Department of Zoology and Anthropology, 949 74, Nitra, Slovak Republic. Electronic address: asirotkin@ukf.sk.
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- $a Földešiová, Martina $u National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Lužianky, Slovak Republic. Electronic address: mt.foldesiova@gmail.com.
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- $a Makovický, Peter $u Czech Centre for Phenogenomics, Division BIOCEV, Institute of Molecular Genetics, Department of Transgenic Models of Diseases, ASCR, 252 50, Vestec, Czech Republic. Electronic address: pmakovicky@email.cz.
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- $a Chrastinová, Ľubica $u National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Lužianky, Slovak Republic. Electronic address: chrastinova@vuzv.sk.
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- $a Makovický, Pavol $u Selye Janos University, Faculty of Education, 945 01, Komárno, Slovak Republic. Electronic address: makovicky.pavol@gmail.cz.
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- $a Chrenek, Peter $u National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Lužianky, Slovak Republic; Slovak University of Agriculture, Faculty of Biotechnology and Food Science, 949 76, Nitra, Slovak Republic; UTP University of Science and Technology, Faculty of Animal Breeding and Biology, Mazowiecka 28, 85-084, Bydgoszcz, Poland. Electronic address: chrenekp@vuzv.sk.
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