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Pellet patented technology for fast and distinct visual detection of cholinesterase inhibitors in liquids

J. Vysloužil, D. Vetchý, J. Zeman, O. Farsa, A. Franc, J. Gajdziok, J. Vysloužil, K. Ficeriová, P. Kulich, Z. Kobliha, V. Pitschmann,

. 2018 ; 161 (-) : 206-213. [pub] 20180825

Language English Country England, Great Britain

Document type Journal Article

The main objective of the presented research was to prepare an innovative carrier as a filler for detection tubes in the form of double-coated pellets with a very significant color transition during the detection of cholinesterase inhibitors such as nerve agents, organophosphorus or carbamate insecticides in liquids that is observable visually and also spectrophotometrically at 412 nm. The pellet cores were prepared by the extrusion/spheronization method. Consecutively, two different coats were applied on the pellet cores in the coating device using the Wurster column method. To increase the color change intensity, the second semipermeable coat based on Eudragit® RL was applied on top of the first coat, which was formed by butyrylcholinesterase immobilized in hydroxypropyl methylcellulose. Prepared samples differing in thickness of the second coat were evaluated for their quality parameters, enzymatic activity and inhibition. The detection mechanism was based on the standard Ellman's colorimetric reaction. It was observed that the semipermeable coat prevented leaching of the enzyme into the solution and led to an increased intensity of color transition from white - yellow to white - deep yellow/orange, thus enabling a more accurate visual detection. This system allows easy, rapid and safe identification of cholinesterase inhibitors in liquids, especially chemical warfare agents.

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$a The main objective of the presented research was to prepare an innovative carrier as a filler for detection tubes in the form of double-coated pellets with a very significant color transition during the detection of cholinesterase inhibitors such as nerve agents, organophosphorus or carbamate insecticides in liquids that is observable visually and also spectrophotometrically at 412 nm. The pellet cores were prepared by the extrusion/spheronization method. Consecutively, two different coats were applied on the pellet cores in the coating device using the Wurster column method. To increase the color change intensity, the second semipermeable coat based on Eudragit® RL was applied on top of the first coat, which was formed by butyrylcholinesterase immobilized in hydroxypropyl methylcellulose. Prepared samples differing in thickness of the second coat were evaluated for their quality parameters, enzymatic activity and inhibition. The detection mechanism was based on the standard Ellman's colorimetric reaction. It was observed that the semipermeable coat prevented leaching of the enzyme into the solution and led to an increased intensity of color transition from white - yellow to white - deep yellow/orange, thus enabling a more accurate visual detection. This system allows easy, rapid and safe identification of cholinesterase inhibitors in liquids, especially chemical warfare agents.
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$a Vetchý, David $u Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, Brno, Czech Republic. Electronic address: vetchy@email.cz.
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$a Zeman, Jiří $7 xx0248510 $u Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, Brno, Czech Republic.
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$a Farsa, Oldřich $u Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, Brno, Czech Republic.
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$a Franc, Aleš $u Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, Brno, Czech Republic.
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$a Gajdziok, Jan $u Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, Brno, Czech Republic.
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$a Vysloužil, Jan $u Department of Biochemistry, Faculty of Science, Masaryk University Brno, Kotlářská 267/2, Brno, Czech Republic.
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$a Ficeriová, Katarína $u Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, Brno, Czech Republic.
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$a Kulich, Pavel $u Department of Chemistry and Toxicology, Veterinary Research Institute Brno, Hudcova 296/70, Brno, Czech Republic.
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$a Kobliha, Zbyněk $u NBC Defence Institute, University of Defence in Brno, Kounicova 65, Brno, Czech Republic.
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$a Pitschmann, Vladimír $u Oritest Ltd., Nábřežní 90/4, Prague, Czech Republic.
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