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Surface functionalization of polyurethane scaffolds mimicking the myocardial microenvironment to support cardiac primitive cells

M. Boffito, F. Di Meglio, P. Mozetic, SM. Giannitelli, I. Carmagnola, C. Castaldo, D. Nurzynska, AM. Sacco, R. Miraglia, S. Montagnani, N. Vitale, M. Brancaccio, G. Tarone, F. Basoli, A. Rainer, M. Trombetta, G. Ciardelli, V. Chiono,

. 2018 ; 13 (7) : e0199896. [pub] 20180706

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19012566

Scaffolds populated with human cardiac progenitor cells (CPCs) represent a therapeutic opportunity for heart regeneration after myocardial infarction. In this work, square-grid scaffolds are prepared by melt-extrusion additive manufacturing from a polyurethane (PU), further subjected to plasma treatment for acrylic acid surface grafting/polymerization and finally grafted with laminin-1 (PU-LN1) or gelatin (PU-G) by carbodiimide chemistry. LN1 is a cardiac niche extracellular matrix component and plays a key role in heart formation during embryogenesis, while G is a low-cost cell-adhesion protein, here used as a control functionalizing molecule. X-ray photoelectron spectroscopy analysis shows nitrogen percentage increase after functionalization. O1s and C1s core-level spectra and static contact angle measurements show changes associated with successful functionalization. ELISA assay confirms LN1 surface grafting. PU-G and PU-LN1 scaffolds both improve CPC adhesion, but LN1 functionalization is superior in promoting proliferation, protection from apoptosis and expression of differentiation markers for cardiomyocytes, endothelial and smooth muscle cells. PU-LN1 and PU scaffolds are biodegraded into non-cytotoxic residues. Scaffolds subcutaneously implanted in mice evoke weak inflammation and integrate with the host tissue, evidencing a significant blood vessel density around the scaffolds. PU-LN1 scaffolds show their superiority in driving CPC behavior, evidencing their promising role in myocardial regenerative medicine.

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$a Di Meglio, Franca $u Department of Public Health, University of Naples 'Federico II', Naples, Italy.
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$a Mozetic, Pamela $u Department of Engineering, Tissue Engineering Unit, Università Campus Bio-Medico di Roma, Rome, Italy. Center for Translational Medicine, International Clinical Research Center, St.Anne's University Hospital, Brno, Czechia.
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$a Giannitelli, Sara Maria $u Department of Engineering, Tissue Engineering Unit, Università Campus Bio-Medico di Roma, Rome, Italy.
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$a Vitale, Nicoletta $u Department of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy.
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