Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma

J. Breza, A. Soltysova, S. Hudecova, A. Penesova, I. Szadvari, P. Babula, B. Chovancova, L. Lencesova, O. Pos, J. Breza, K. Ondrias, O. Krizanova,

. 2018 ; 18 (1) : 591. [pub] 20180524

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19012652

BACKGROUND: Knowledge about the expression and thus a role of enzymes that produce endogenous H2S - cystathionine-β-synthase, cystathionine γ-lyase and mercaptopyruvate sulfurtransferase - in renal tumors is still controversial. In this study we aimed to determine the expression of these enzymes relatively to the expression in unaffected part of kidney from the same patient and to found relation of these changes to apoptosis. To evaluate patient's samples, microarray and immunohistochemistry was used. METHODS: To determine the physiological importance, we used RCC4 stable cell line derived from clear cell renal cell carcinoma, where apoptosis induction by a mixture of five chemotherapeutics with/without silencing of H2S-producing enzymes was detected. Immunofluorescence was used to determine each enzyme in the cells. RESULTS: In clear cell renal cell carcinomas, expression of H2S-producing enzymes was mostly decreased compared to a part of kidney that was distal from the tumor. To evaluate a potential role of H2S-producing enzymes in the apoptosis induction, we used RCC4 stable cell line. We have found that silencing of cystathionine-β-synthase and cystathionine γ-lyase prevented induction of apoptosis. Immunofluorescence staining clearly showed that these enzymes were upregulated during apoptosis in RCC4 cells. CONCLUSION: Based on these results we concluded that in clear cell renal cell carcinoma, reduced expression of the H2S-producing enzymes, mainly cystathionine γ-lyase, might contribute to a resistance to the induction of apoptosis. Increased production of the endogenous H2S, or donation from the external sources might be of a therapeutic importance in these tumors.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19012652
003      
CZ-PrNML
005      
20230718120347.0
007      
ta
008      
190405s2018 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1186/s12885-018-4508-1 $2 doi
035    __
$a (PubMed)29793450
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Breza, Jan $u Department of Urology with Kidney Transplant Center, University Hospital, Faculty of Medicine, Bratislava, Slovakia.
245    10
$a Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma / $c J. Breza, A. Soltysova, S. Hudecova, A. Penesova, I. Szadvari, P. Babula, B. Chovancova, L. Lencesova, O. Pos, J. Breza, K. Ondrias, O. Krizanova,
520    9_
$a BACKGROUND: Knowledge about the expression and thus a role of enzymes that produce endogenous H2S - cystathionine-β-synthase, cystathionine γ-lyase and mercaptopyruvate sulfurtransferase - in renal tumors is still controversial. In this study we aimed to determine the expression of these enzymes relatively to the expression in unaffected part of kidney from the same patient and to found relation of these changes to apoptosis. To evaluate patient's samples, microarray and immunohistochemistry was used. METHODS: To determine the physiological importance, we used RCC4 stable cell line derived from clear cell renal cell carcinoma, where apoptosis induction by a mixture of five chemotherapeutics with/without silencing of H2S-producing enzymes was detected. Immunofluorescence was used to determine each enzyme in the cells. RESULTS: In clear cell renal cell carcinomas, expression of H2S-producing enzymes was mostly decreased compared to a part of kidney that was distal from the tumor. To evaluate a potential role of H2S-producing enzymes in the apoptosis induction, we used RCC4 stable cell line. We have found that silencing of cystathionine-β-synthase and cystathionine γ-lyase prevented induction of apoptosis. Immunofluorescence staining clearly showed that these enzymes were upregulated during apoptosis in RCC4 cells. CONCLUSION: Based on these results we concluded that in clear cell renal cell carcinoma, reduced expression of the H2S-producing enzymes, mainly cystathionine γ-lyase, might contribute to a resistance to the induction of apoptosis. Increased production of the endogenous H2S, or donation from the external sources might be of a therapeutic importance in these tumors.
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    12
$a apoptóza $7 D017209
650    _2
$a karcinom z renálních buněk $x patologie $x chirurgie $7 D002292
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a cystathionin-beta-synthasa $x genetika $x metabolismus $7 D003541
650    _2
$a cystathionin-gama-lyasa $x genetika $x metabolismus $7 D003542
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a sulfan $x metabolismus $7 D006862
650    _2
$a ledviny $x metabolismus $x patologie $x chirurgie $7 D007668
650    _2
$a nádory ledvin $x patologie $x chirurgie $7 D007680
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a lidé středního věku $7 D008875
650    _2
$a nefrektomie $7 D009392
650    _2
$a RNA interference $7 D034622
650    _2
$a malá interferující RNA $x metabolismus $7 D034741
650    _2
$a upregulace $7 D015854
655    _2
$a časopisecké články $7 D016428
700    1_
$a Soltysova, Andrea $u Institute of Clinical and Translational Research, Biomedical Research Center, SAS, Bratislava, Slovakia. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
700    1_
$a Hudecova, Sona $u Institute of Clinical and Translational Research, Biomedical Research Center, SAS, Bratislava, Slovakia.
700    1_
$a Penesova, Adela $u Institute of Clinical and Translational Research, Biomedical Research Center, SAS, Bratislava, Slovakia.
700    1_
$a Szadvári, Ivan, $u Department of Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. $d 1985- $7 xx0304142
700    1_
$a Babula, Petr $u Department of Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Chovancova, Barbora $u Institute of Clinical and Translational Research, Biomedical Research Center, SAS, Bratislava, Slovakia.
700    1_
$a Lencesova, Lubomira $u Institute of Clinical and Translational Research, Biomedical Research Center, SAS, Bratislava, Slovakia.
700    1_
$a Pos, Ondrej $u Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
700    1_
$a Breza, Jan $u Department of Urology with Kidney Transplant Center, University Hospital, Faculty of Medicine, Bratislava, Slovakia.
700    1_
$a Ondrias, Karol $u Institute of Clinical and Translational Research, Biomedical Research Center, SAS, Bratislava, Slovakia.
700    1_
$a Krizanova, Olga $u Institute of Clinical and Translational Research, Biomedical Research Center, SAS, Bratislava, Slovakia. olga.krizanova@savba.sk. Department of Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. olga.krizanova@savba.sk.
773    0_
$w MED00008171 $t BMC cancer $x 1471-2407 $g Roč. 18, č. 1 (2018), s. 591
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29793450 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190405 $b ABA008
991    __
$a 20230718120344 $b ABA008
999    __
$a ok $b bmc $g 1391962 $s 1050957
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 18 $c 1 $d 591 $e 20180524 $i 1471-2407 $m BMC cancer $n BMC Cancer $x MED00008171
LZP    __
$a Pubmed-20190405
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace