-
Je něco špatně v tomto záznamu ?
GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol
P. Hušek, Z. Švagera, D. Hanzlíková, I. Karlínová, L. Řimnáčová, H. Zahradníčková, P. Šimek,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- analýza moči metody MeSH
- biologické markery moč MeSH
- fluorokarbony chemie MeSH
- formiáty chemie MeSH
- lidé MeSH
- metabolomika metody MeSH
- mikroextrakce kapalné fáze metody MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A simple analytical workflow is described for gas chromatographic-mass spectrometry (GC-MS)-based metabolomic profiling of protic metabolites, particularly amino-carboxylic species in biological matrices. The sample preparation is carried out directly in aqueous samples and uses simultaneous in situ heptafluorobutyl chloroformate (HFBCF) derivatization and dispersive liquid-liquid microextraction (DLLME), followed by GC-MS analysis in single-ion monitoring (SIM) mode. The protocol involves ten simple pipetting steps and provides quantitative analysis of 132 metabolites by using two internal standards. A comment on each analytical step and explaining notes are provided with particular attention to the GC-MS analysis of 112 physiological metabolites in human urine.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19012724
- 003
- CZ-PrNML
- 005
- 20190405100304.0
- 007
- ta
- 008
- 190405s2018 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/978-1-4939-7643-0_11 $2 doi
- 035 __
- $a (PubMed)29654589
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Hušek, Petr $u University Hospital Ostrava, Institute of Laboratory Diagnostics, Department of Biochemistry, Ostrava, Czech Republic. Czech Academy of Sciences, Biology Centre, Institute of Entomology, Analytical Biochemistry & Metabolomics, České Budějovice, Czech Republic.
- 245 10
- $a GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol / $c P. Hušek, Z. Švagera, D. Hanzlíková, I. Karlínová, L. Řimnáčová, H. Zahradníčková, P. Šimek,
- 520 9_
- $a A simple analytical workflow is described for gas chromatographic-mass spectrometry (GC-MS)-based metabolomic profiling of protic metabolites, particularly amino-carboxylic species in biological matrices. The sample preparation is carried out directly in aqueous samples and uses simultaneous in situ heptafluorobutyl chloroformate (HFBCF) derivatization and dispersive liquid-liquid microextraction (DLLME), followed by GC-MS analysis in single-ion monitoring (SIM) mode. The protocol involves ten simple pipetting steps and provides quantitative analysis of 132 metabolites by using two internal standards. A comment on each analytical step and explaining notes are provided with particular attention to the GC-MS analysis of 112 physiological metabolites in human urine.
- 650 _2
- $a biologické markery $x moč $7 D015415
- 650 _2
- $a fluorokarbony $x chemie $7 D005466
- 650 _2
- $a formiáty $x chemie $7 D005561
- 650 _2
- $a plynová chromatografie s hmotnostně spektrometrickou detekcí $x metody $7 D008401
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mikroextrakce kapalné fáze $x metody $7 D059627
- 650 _2
- $a metabolomika $x metody $7 D055432
- 650 _2
- $a analýza moči $x metody $7 D016482
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Švagera, Zdeněk $u University Hospital Ostrava, Institute of Laboratory Diagnostics, Department of Biochemistry, Ostrava, Czech Republic.
- 700 1_
- $a Hanzlíková, Dagmar $u University Hospital Ostrava, Institute of Laboratory Diagnostics, Department of Biochemistry, Ostrava, Czech Republic.
- 700 1_
- $a Karlínová, Iva $u Czech Academy of Sciences, Biology Centre, Institute of Entomology, Analytical Biochemistry & Metabolomics, České Budějovice, Czech Republic.
- 700 1_
- $a Řimnáčová, Lucie $u Czech Academy of Sciences, Biology Centre, Institute of Entomology, Analytical Biochemistry & Metabolomics, České Budějovice, Czech Republic.
- 700 1_
- $a Zahradníčková, Helena $u Czech Academy of Sciences, Biology Centre, Institute of Entomology, Analytical Biochemistry & Metabolomics, České Budějovice, Czech Republic.
- 700 1_
- $a Šimek, Petr $u Czech Academy of Sciences, Biology Centre, Institute of Entomology, Analytical Biochemistry & Metabolomics, České Budějovice, Czech Republic. simek@bclab.eu.
- 773 0_
- $w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 1738, č. - (2018), s. 159-181
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29654589 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190405 $b ABA008
- 991 __
- $a 20190405100314 $b ABA008
- 999 __
- $a ok $b bmc $g 1392034 $s 1051029
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 1738 $c - $d 159-181 $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
- LZP __
- $a Pubmed-20190405
