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GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol
P. Hušek, Z. Švagera, D. Hanzlíková, I. Karlínová, L. Řimnáčová, H. Zahradníčková, P. Šimek,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Urinalysis methods MeSH
- Biomarkers urine MeSH
- Fluorocarbons chemistry MeSH
- Formates chemistry MeSH
- Humans MeSH
- Metabolomics methods MeSH
- Liquid Phase Microextraction methods MeSH
- Gas Chromatography-Mass Spectrometry methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A simple analytical workflow is described for gas chromatographic-mass spectrometry (GC-MS)-based metabolomic profiling of protic metabolites, particularly amino-carboxylic species in biological matrices. The sample preparation is carried out directly in aqueous samples and uses simultaneous in situ heptafluorobutyl chloroformate (HFBCF) derivatization and dispersive liquid-liquid microextraction (DLLME), followed by GC-MS analysis in single-ion monitoring (SIM) mode. The protocol involves ten simple pipetting steps and provides quantitative analysis of 132 metabolites by using two internal standards. A comment on each analytical step and explaining notes are provided with particular attention to the GC-MS analysis of 112 physiological metabolites in human urine.
References provided by Crossref.org
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