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Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development
T. Vain, S. Raggi, N. Ferro, DK. Barange, M. Kieffer, Q. Ma, SM. Doyle, M. Thelander, B. Pařízková, O. Novák, A. Ismail, PA. Enquist, A. Rigal, M. Łangowska, S. Ramans Harborough, Y. Zhang, K. Ljung, J. Callis, F. Almqvist, S. Kepinski, M....
Language English Country United States
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
Grant support
BB/L010623/1
Biotechnology and Biological Sciences Research Council - United Kingdom
NLK
Free Medical Journals
from 1915 to 6 months ago
Freely Accessible Science Journals
from 1915 to 6 months ago
PubMed Central
from 1915 to 6 months ago
Europe PubMed Central
from 1915 to 6 months ago
Open Access Digital Library
from 1915-01-15
Open Access Digital Library
from 1915-01-01
- MeSH
- Arabidopsis genetics metabolism MeSH
- F-Box Proteins metabolism MeSH
- Transcription, Genetic drug effects MeSH
- Plants, Genetically Modified genetics MeSH
- Indoleacetic Acids metabolism MeSH
- NEDD8 Protein genetics MeSH
- SKP Cullin F-Box Protein Ligases metabolism MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Proteolysis * MeSH
- Receptors, Cell Surface metabolism MeSH
- Gene Expression Regulation, Plant MeSH
- Plant Growth Regulators genetics metabolism MeSH
- Seedlings metabolism MeSH
- Signal Transduction MeSH
- Transcription Factors metabolism MeSH
- Plant Development genetics physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Auxin phytohormones control most aspects of plant development through a complex and interconnected signaling network. In the presence of auxin, AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors are targeted for degradation by the SKP1-CULLIN1-F-BOX (SCF) ubiquitin-protein ligases containing TRANSPORT INHIBITOR RESISTANT 1/AUXIN SIGNALING F-BOX (TIR1/AFB). CULLIN1-neddylation is required for SCFTIR1/AFB functionality, as exemplified by mutants deficient in the NEDD8-activating enzyme subunit AUXIN-RESISTANT 1 (AXR1). Here, we report a chemical biology screen that identifies small molecules requiring AXR1 to modulate plant development. We selected four molecules of interest, RubNeddin 1 to 4 (RN1 to -4), among which RN3 and RN4 trigger selective auxin responses at transcriptional, biochemical, and morphological levels. This selective activity is explained by their ability to consistently promote the interaction between TIR1 and a specific subset of AUX/IAA proteins, stimulating the degradation of particular AUX/IAA combinations. Finally, we performed a genetic screen using RN4, the RN with the greatest potential for dissecting auxin perception, which revealed that the chromatin remodeling ATPase BRAHMA is implicated in auxin-mediated apical hook development. These results demonstrate the power of selective auxin agonists to dissect auxin perception for plant developmental functions, as well as offering opportunities to discover new molecular players involved in auxin responses.
Centre for Plant Sciences University of Leeds LS2 9JT Leeds United Kingdom
Department of Molecular and Cellular Biology University of California Davis CA 95616
Institute of Physical and Theoretical Chemistry University of Bonn 53121 Bonn Germany
Section of Cell and Developmental Biology University of California San Diego La Jolla CA 92093 0116
References provided by Crossref.org
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- $a Auxin phytohormones control most aspects of plant development through a complex and interconnected signaling network. In the presence of auxin, AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors are targeted for degradation by the SKP1-CULLIN1-F-BOX (SCF) ubiquitin-protein ligases containing TRANSPORT INHIBITOR RESISTANT 1/AUXIN SIGNALING F-BOX (TIR1/AFB). CULLIN1-neddylation is required for SCFTIR1/AFB functionality, as exemplified by mutants deficient in the NEDD8-activating enzyme subunit AUXIN-RESISTANT 1 (AXR1). Here, we report a chemical biology screen that identifies small molecules requiring AXR1 to modulate plant development. We selected four molecules of interest, RubNeddin 1 to 4 (RN1 to -4), among which RN3 and RN4 trigger selective auxin responses at transcriptional, biochemical, and morphological levels. This selective activity is explained by their ability to consistently promote the interaction between TIR1 and a specific subset of AUX/IAA proteins, stimulating the degradation of particular AUX/IAA combinations. Finally, we performed a genetic screen using RN4, the RN with the greatest potential for dissecting auxin perception, which revealed that the chromatin remodeling ATPase BRAHMA is implicated in auxin-mediated apical hook development. These results demonstrate the power of selective auxin agonists to dissect auxin perception for plant developmental functions, as well as offering opportunities to discover new molecular players involved in auxin responses.
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