A compromised detection of radiation-induced plasmid DNA fragments results in underestimation of calculated damage yields. Electrophoretic methods are easy and cheap, but they can only detect a part of the fragments, neglecting the shortest ones. These can be detected with atomic force microscopy, but at the expense of time and price. Both methods were used to investigate their capabilities to detect the DNA fragments induced by high-energetic heavy ions. The results were taken into account in calculations of radiation-induced yields of single and double strand breaks. It was estimated that the double strand break yield is twice as high when the fragments are at least partially detected with the agarose electrophoresis, compared to when they were completely omitted. Further increase by 13% was observed when the measured fragments were corrected for the fraction of the shortest fragments up to 300 base pairs, as detected with the atomic force microscopy. The effect of fragment detection on the single strand break yield was diminished.

Atominstitut Technische Universität Wien Stadionallee 2 Wien Austria MedAustron Marie Curie Straße 5 Wiener Neustadt Austria

Department of Radiation Dosimetry Nuclear Physics Institute of the Czech Academy of Sciences Na Truhlářce 39 64 Prague Czech Republic

Department of Thin Films and Nanostructures Institute of Physics of the Czech Academy of Sciences Cukrovarnická 10 112 Prague Czech Republic

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