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Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (2015-mid 2018)

S. Štěpánová, V. Kašička,

. 2019 ; 42 (1) : 398-414. [pub] 20181128

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, přehledy

Perzistentní odkaz   https://www.medvik.cz/link/bmc19028059

Grantová podpora
17-10832S Czech Science Foundation
18-02597S Czech Science Foundation
RVO 61388963 Czech Academy of Sciences

This review summarizes recent developments and applications of capillary and microchip electroseparation methods in proteomic and peptidomic analyses since the year 2015 to ca. mid 2018. Sample preparation procedures for the removal of interfering components or for pre-fractionation and preconcentration of proteins and peptides of interest are discussed. The innovations in coupling of capillary or microchip electroseparation methods with different modes of mass spectrometry detection are covered. In addition, significant recent applications of capillary electromigration methods in both bottom-up and top-down proteomics as well as in determinations of post-translational modifications of proteins are presented. Moreover, several examples of the utilization of capillary electromigration methods coupled with mass spectrometry detection for clinical proteomics and peptidomics are described.

Citace poskytuje Crossref.org

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