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Profiling system for skin kallikrein proteolysis applied in gene-deficient mouse models

M. Horn, O. Zbodakova, P. Kasparek, J. Srp, R. Haneckova, M. Hradilek, M. Mares, R. Sedlacek,

. 2018 ; 399 (9) : 1085-1089. [pub] 20180925

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19028388

Kallikrein-related proteases (KLKs) play a critical role in epidermis physiology and have been implicated in skin pathologies such as Netherton syndrome. The contribution of individual KLKs to skin proteolysis is poorly understood. Monitoring of their activities in skin proteome is hampered by overlapping substrate specificities, and there is a need for novel assays. Here, we present a platform of selective and sensitive fluorogenic substrates and inhibitors for profiling KLK5, KLK7 and KLK14. These chemical tools were evaluated using recombinant KLKs and tissue from a unique set of mice deficient in eight combinations of KLKs and their natural regulator LEKTI.

Citace poskytuje Crossref.org

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$a Kasparek, Petr $u Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the ASCR, Prumyslova 595, 252 50 Vestec, Czech Republic. Czech Centre for Phenogenomics, Institute of Molecular Genetics of the ASCR, Prumyslova 595, 252 50 Vestec, Czech Republic.
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$a Srp, Jaroslav $u Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo n. 2, 166 10 Prague, Czech Republic. Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030/8, 128 00 Prague, Czech Republic.
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$a Haneckova, Radka $u Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the ASCR, Prumyslova 595, 252 50 Vestec, Czech Republic.
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$a Hradilek, Martin $u Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo n. 2, 166 10 Prague, Czech Republic.
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$a Sedlacek, Radislav $u Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the ASCR, Prumyslova 595, 252 50 Vestec, Czech Republic. Czech Centre for Phenogenomics, Institute of Molecular Genetics of the ASCR, Prumyslova 595, 252 50 Vestec, Czech Republic.
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