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Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer
J. Remšík, R. Fedr, J. Navrátil, L. Binó, E. Slabáková, P. Fabian, M. Svoboda, K. Souček,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NV15-33999A
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
Free Medical Journals
from 1947 to 1 year ago
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from 1947 to 1 year ago
PubMed Central
from 1947 to 1 year ago
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from 1947 to 1 year ago
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from 2000-01-01 to 1 year ago
Open Access Digital Library
from 1947-01-01
Open Access Digital Library
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Nursing & Allied Health Database (ProQuest)
from 2000-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2000-01-01 to 1 year ago
Public Health Database (ProQuest)
from 2000-01-01 to 1 year ago
PubMed
29462126
DOI
10.1038/bjc.2017.497
Knihovny.cz E-resources
- MeSH
- Tetraspanin 29 biosynthesis immunology MeSH
- Antigens, Neoplasm biosynthesis immunology MeSH
- Antigens, Surface biosynthesis immunology MeSH
- Epithelial-Mesenchymal Transition immunology MeSH
- Transcription, Genetic MeSH
- Humans MeSH
- Neoplasm Metastasis MeSH
- Biomarkers, Tumor MeSH
- Cell Line, Tumor MeSH
- Breast Neoplasms genetics immunology pathology MeSH
- Cell Plasticity immunology MeSH
- Cellular Reprogramming physiology MeSH
- Flow Cytometry MeSH
- High-Throughput Screening Assays MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Background:The intratumoural heterogeneity, often driven by epithelial-to-mesenchymal transition (EMT), significantly contributes to chemoresistance and disease progression in adenocarcinomas. Methods:We introduced a high-throughput screening platform to identify surface antigens that associate with epithelial–mesenchymal plasticity in well-defined pairs of epithelial cell lines and their mesenchymal counterparts. Using multicolour flow cytometry, we then analysed the expression of 10 most robustly changed antigens and identified a 10-molecule surface signature, in pan-cytokeratin-positive/EpCAM-positive and -negative fractions of dissociated breast tumours. Results:We found that surface CD9, CD29, CD49c, and integrin ß5 are lost in breast cancer cells that underwent EMT in vivo. The tetraspanin family member CD9 was concordantly downregulated both in vitro and in vivo and associated with epithelial phenotype and favourable prognosis. Conclusions:We propose that overall landscape of 10-molecule surface signature expression reflects the epithelial–mesenchymal plasticity in breast cancer.
References provided by Crossref.org
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