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Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence-A Transcriptomic Study

B. Chermuła, M. Brązert, M. Jeseta, K. Ożegowska, I. Kocherova, M. Jankowski, P. Celichowski, P. Sujka-Kordowska, A. Konwerska, H. Piotrowska-Kempisty, J. Budna-Tukan, P. Antosik, D. Bukowska, M. Machatkova, KP. Brussow, MT. Skowroński, L....

. 2019 ; 20 (9) : . [pub] 20190507

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19034581

Grantová podpora
UMO-2016/21/B/NZ9/03535 Narodowe Centrum Nauki

This paper aims to identify and describe new genetic markers involved in the processes of protein expression and modification reflected in the change of mitochondrial activity before and after in vitro maturation of the oocyte. Porcine oocytes collected from the ovaries of slaughtered landrace gilts were subjected to the process of in vitro maturation. Transcriptomic changes in the expression profile of oocyte genes involved in response to hypoxia, the transmembrane protein receptor serine threonine kinase signaling pathway, the "transforming growth factor β receptor signaling pathway", "response to protein stimulus", and "response to organic substance" were investigated using microarrays. The expression values of these genes in oocytes was analyzed before (immature) and after (mature) in vitro maturation, with significant differences found. All the significantly altered genes showed downregulation after the maturation process. The most changed genes from these gene ontologies, FOS, ID2, VEGFA, BTG2, CYR61, ESR1, AR, TACR3, CCND2, CHRDL1, were chosen to be further validated, described and related to the literature. Additionally, the mitochondrial activity of the analyzed oocytes was measured using specific dyes. We found that the mitochondrial activity was higher before the maturation process. The analysis of these results and the available literature provides a novel insight on the processes that occur during in vitro oocyte maturation. While this knowledge may prove to be useful in further research of the procedures commonly associated with in vitro fertilization procedures, it serves mostly as a basic reference for further proteomic, in vivo, and clinical studies that are necessary to translate it into practical applications.

Citace poskytuje Crossref.org

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$a Chermuła, Błażej $u Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60-535 Poznań, Poland. blazej.chermula@wp.pl.
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$a This paper aims to identify and describe new genetic markers involved in the processes of protein expression and modification reflected in the change of mitochondrial activity before and after in vitro maturation of the oocyte. Porcine oocytes collected from the ovaries of slaughtered landrace gilts were subjected to the process of in vitro maturation. Transcriptomic changes in the expression profile of oocyte genes involved in response to hypoxia, the transmembrane protein receptor serine threonine kinase signaling pathway, the "transforming growth factor β receptor signaling pathway", "response to protein stimulus", and "response to organic substance" were investigated using microarrays. The expression values of these genes in oocytes was analyzed before (immature) and after (mature) in vitro maturation, with significant differences found. All the significantly altered genes showed downregulation after the maturation process. The most changed genes from these gene ontologies, FOS, ID2, VEGFA, BTG2, CYR61, ESR1, AR, TACR3, CCND2, CHRDL1, were chosen to be further validated, described and related to the literature. Additionally, the mitochondrial activity of the analyzed oocytes was measured using specific dyes. We found that the mitochondrial activity was higher before the maturation process. The analysis of these results and the available literature provides a novel insight on the processes that occur during in vitro oocyte maturation. While this knowledge may prove to be useful in further research of the procedures commonly associated with in vitro fertilization procedures, it serves mostly as a basic reference for further proteomic, in vivo, and clinical studies that are necessary to translate it into practical applications.
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$a Brązert, Maciej $u Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60-535 Poznań, Poland. maciejbrazert@ump.edu.pl.
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$a Jeseta, Michal $u Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 601 77 Brno, Czech Republic. jeseta@gmail.com.
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$a Ożegowska, Katarzyna $u Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60-535 Poznań, Poland. katarzyna.ozegowska@ump.edu.pl.
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$a Kocherova, Ievgenia $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. kocherova.evgenia@gmail.com.
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$a Jankowski, Maurycy $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. m.jankowski.14@aberdeen.ac.uk.
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$a Celichowski, Piotr $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland. p.celichowski@gmail.com.
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$a Piotrowska-Kempisty, Hanna $u Department of Toxicology, Poznan University of Medical Sciences, 61-631 Poznań, Poland. hpiotrow@ump.edu.pl.
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$a Budna-Tukan, Joanna $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland. joanna.budna@wp.pl.
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$a Antosik, Paweł $u Veterinary Center, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland. pantosik@umk.pl.
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$a Bukowska, Dorota $u Veterinary Center, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland. dbukowska@umk.pl.
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$a Machatkova, Marie $u Veterinary Research Institute, 621 00 Brno, Czech Republic. machatkova@vri.cz.
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$a Brussow, Klaus P $u Veterinary Center, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland. prof.bruessow@gmail.com.
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$a Pawelczyk, Leszek $u Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60-535 Poznań, Poland. pawelczyk.leszek@ump.edu.pl.
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