BACKGROUND: Many transfusion services determine the titer of potentially incompatible plasma-containing products by performing a one-dilution titer at their selected titer threshold. This study compared the results of immediate spin (IS) one-dilution titers determined by three methods with a reference standard method. METHODS: Plasma-containing products from group A and O donors were titered using the participant's routine IS one-dilution titer method. No time or temperature incubations were performed, and antihuman globulin reagent was not used. The samples were then tested using a reference method, which was a saline tube test with a 1-hour room temperature incubation; antihuman globulin was not used in the reference method. The results of the one-dilution titer were then compared to that obtained in the reference method. RESULTS: Nine centers participated in this study. There were 698 antibodies from 374 units tested by the manual IS tube one-dilution titer method; sensitivity was 0.88 (95% confidence interval [CI], 0.83-0.92), and specificity was 1.00 (95% CI, 0.98-1.00). There were 412 antibodies from 206 units tested by the manual and automated IS buffered gel card one-dilution titer method; sensitivity was 0.95 (95% CI, 0.91-0.98), and specificity was 0.87 (95% CI, 0.81-0.91). There were 98 antibodies from 49 units tested by an automated microplate IS one-dilution titer method; sensitivity was 0.76 (95% CI, 0.71-0.93), and specificity was 0.96 (95% CI, 0.92-0.99). All three methods had an accuracy rate of 90% or greater. CONCLUSION: The manual and automated one-dilution titer methods are suitable for screening plasma-containing units, although more evaluation of the automated microplate method might be required.

Blood Transfusion and Tissue Department University Hospital Brno Brno Czech Republic

Blood Transfusion Department Regional Hospital Karlovy Vary Prague Czech Republic

Department of Hematology and Blood Transfusion Military University Hospital Prague Prague Czech Republic

Department of Hematology and Blood Transfusion Regional Hospital Liberec Liberec Czech Republic

Department of Hemotherapy and Cell Terapy Hospital Israelita Albert Einstein Sao Paulo Brazil

Department of Pathology and Laboratory Medicine Dartmouth Hitchcock Medical Center Lebanon New Hampshire

Department of Pathology and Laboratory Medicine University of North Carolina Chapel Hill North Carolina

Department of Pathology University of Pittsburgh Pittsburgh Pennsylvania The Institute for Transfusion Medicine Pittsburgh Pennsylvania

Wing Kwai and Alice Lee Tsing Chung Transfusion Service Department of Pathology and Laboratory Medicine UCLA Health Los Angeles California

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$a Yazer, Mark H $u Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania. The Institute for Transfusion Medicine, Pittsburgh, Pennsylvania.

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$a Comparison of titer results obtained using immediate spin one-dilution techniques to a reference method / $c MH. Yazer, NM. Dunbar, CB. Bub, BE. Condict, R. Dunn, M. Janoušková, J. Kutner, L. Landová, H. Lejdarová, B. Nesvačilová, R. Pacasová, R. Procházková, L. Řehořová, JS. Raval, A. Ziman, M. Bohonĕk,

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$a BACKGROUND: Many transfusion services determine the titer of potentially incompatible plasma-containing products by performing a one-dilution titer at their selected titer threshold. This study compared the results of immediate spin (IS) one-dilution titers determined by three methods with a reference standard method. METHODS: Plasma-containing products from group A and O donors were titered using the participant's routine IS one-dilution titer method. No time or temperature incubations were performed, and antihuman globulin reagent was not used. The samples were then tested using a reference method, which was a saline tube test with a 1-hour room temperature incubation; antihuman globulin was not used in the reference method. The results of the one-dilution titer were then compared to that obtained in the reference method. RESULTS: Nine centers participated in this study. There were 698 antibodies from 374 units tested by the manual IS tube one-dilution titer method; sensitivity was 0.88 (95% confidence interval [CI], 0.83-0.92), and specificity was 1.00 (95% CI, 0.98-1.00). There were 412 antibodies from 206 units tested by the manual and automated IS buffered gel card one-dilution titer method; sensitivity was 0.95 (95% CI, 0.91-0.98), and specificity was 0.87 (95% CI, 0.81-0.91). There were 98 antibodies from 49 units tested by an automated microplate IS one-dilution titer method; sensitivity was 0.76 (95% CI, 0.71-0.93), and specificity was 0.96 (95% CI, 0.92-0.99). All three methods had an accuracy rate of 90% or greater. CONCLUSION: The manual and automated one-dilution titer methods are suitable for screening plasma-containing units, although more evaluation of the automated microplate method might be required.

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$a Dunbar, Nancy M $u Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire.

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$a Bub, Carolina Bonet $u Department of Hemotherapy and Cell Terapy, Hospital Israelita Albert Einstein, Sao Paulo, Brazil.

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$a Condict, Brittany E $u Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire.

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$a Dunn, Robyn $u Wing-Kwai and Alice Lee-Tsing Chung Transfusion Service, Department of Pathology and Laboratory Medicine, UCLA Health, Los Angeles, California.

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$a Janoušková, Miloslava $u Blood Transfusion Department, Regional Hospital Karlovy Vary, Prague, Czech Republic.

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$a Kutner, Jose $u Department of Hemotherapy and Cell Terapy, Hospital Israelita Albert Einstein, Sao Paulo, Brazil.

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$a Landová, Ludmila $u Department of Hematology and Blood Transfusion, Military University Hospital Prague, Prague, Czech Republic.

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$a Lejdarová, Hana $u Blood Transfusion and Tissue Department, University Hospital Brno, Brno, Czech Republic.

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$a Nesvačilová, Božena $u Blood Transfusion Department, Regional Hospital Karlovy Vary, Prague, Czech Republic.

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$a Pacasová, Rita $u Blood Transfusion and Tissue Department, University Hospital Brno, Brno, Czech Republic.

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$a Procházková, Renata $u Department of Hematology and Blood Transfusion, Regional Hospital Liberec, Liberec, Czech Republic.

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$a Řehořová, Lenka $u Department of Hematology and Blood Transfusion, Regional Hospital Liberec, Liberec, Czech Republic.

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$a Raval, Jay S $u Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina.

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$a Ziman, Alyssa $u Wing-Kwai and Alice Lee-Tsing Chung Transfusion Service, Department of Pathology and Laboratory Medicine, UCLA Health, Los Angeles, California.

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$a Bohonĕk, Miloš $u Department of Hematology and Blood Transfusion, Military University Hospital Prague, Prague, Czech Republic.

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