Butyrate produced by the intestinal microbiota is essential for proper functioning of the intestinal immune system. Total dependence on parenteral nutrition (PN) is associated with numerous adverse effects, including severe microbial dysbiosis and loss of important butyrate producers. We hypothesised that a lack of butyrate produced by the gut microbiota may be compensated by its supplementation in PN mixtures. We tested whether i.v. butyrate administration would (a) positively modulate intestinal defence mechanisms and (b) counteract PN-induced dysbiosis. Male Wistar rats were randomised to chow, PN, and PN supplemented with 9 mM butyrate (PN+But) for 12 days. Antimicrobial peptides, mucins, tight junction proteins, and cytokine expression were assessed by RT-qPCR. T-cell subpopulations in mesenteric lymph nodes (MLN) were analysed by flow cytometry. Microbiota composition was assessed in caecum content. Butyrate supplementation resulted in increased expression of tight junction proteins (ZO-1, claudin-7, E-cadherin), antimicrobial peptides (Defa 8, Rd5, RegIIIγ), and lysozyme in the ileal mucosa. Butyrate partially alleviated PN-induced intestinal barrier impairment and normalised IL-4, IL-10, and IgA mRNA expression. PN administration was associated with an increase in Tregs in MLN, which was normalised by butyrate. Butyrate increased the total number of CD4+ and decreased a relative amount of CD8+ memory T cells in MLN. Lack of enteral nutrition and PN administration led to a shift in caecal microbiota composition. Butyrate did not reverse the altered expression of most taxa but did influence the abundance of some potentially beneficial/pathogenic genera, which might contribute to its overall beneficial effect.

Clinical and Transplant Pathology Department Institute for Clinical and Experimental Medicine Prague 4 140 21 Czech Republic

Department of Experimental Medicine Institute for Clinical and Experimental Medicine Prague 4 140 21 Czech Republic

Faculty of Forestry and Wood Sciences Czech University of Life Sciences Prague Czech Republic

Prevedig Prague 1 110 00 Czech Republic

RECETOX Faculty of Science Masaryk University Brno 625 00 Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc19034668

003      

CZ-PrNML

005      

20201023113914.0

007      

ta

008      

191007s2019 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1155/2019/7084734 $2 doi

035    __

$a (PubMed)30941370

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxu

100    1_

$a Jirsova, Zuzana $u Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague 4-140 21, Czech Republic.

245    14

$a The Effect of Butyrate-Supplemented Parenteral Nutrition on Intestinal Defence Mechanisms and the Parenteral Nutrition-Induced Shift in the Gut Microbiota in the Rat Model / $c Z. Jirsova, M. Heczkova, H. Dankova, H. Malinska, P. Videnska, H. Vespalcova, L. Micenkova, L. Bartonova, E. Sticova, A. Lodererova, L. Prefertusová, A. Sekerkova, J. Hradecky, M. Cahova,

520    9_

$a Butyrate produced by the intestinal microbiota is essential for proper functioning of the intestinal immune system. Total dependence on parenteral nutrition (PN) is associated with numerous adverse effects, including severe microbial dysbiosis and loss of important butyrate producers. We hypothesised that a lack of butyrate produced by the gut microbiota may be compensated by its supplementation in PN mixtures. We tested whether i.v. butyrate administration would (a) positively modulate intestinal defence mechanisms and (b) counteract PN-induced dysbiosis. Male Wistar rats were randomised to chow, PN, and PN supplemented with 9 mM butyrate (PN+But) for 12 days. Antimicrobial peptides, mucins, tight junction proteins, and cytokine expression were assessed by RT-qPCR. T-cell subpopulations in mesenteric lymph nodes (MLN) were analysed by flow cytometry. Microbiota composition was assessed in caecum content. Butyrate supplementation resulted in increased expression of tight junction proteins (ZO-1, claudin-7, E-cadherin), antimicrobial peptides (Defa 8, Rd5, RegIIIγ), and lysozyme in the ileal mucosa. Butyrate partially alleviated PN-induced intestinal barrier impairment and normalised IL-4, IL-10, and IgA mRNA expression. PN administration was associated with an increase in Tregs in MLN, which was normalised by butyrate. Butyrate increased the total number of CD4+ and decreased a relative amount of CD8+ memory T cells in MLN. Lack of enteral nutrition and PN administration led to a shift in caecal microbiota composition. Butyrate did not reverse the altered expression of most taxa but did influence the abundance of some potentially beneficial/pathogenic genera, which might contribute to its overall beneficial effect.

650    _2

$a zvířata $7 D000818

650    _2

$a biodiverzita $7 D044822

650    _2

$a butyráty $x farmakologie $7 D002087

650    _2

$a kolon $x účinky léků $x patologie $7 D003106

650    12

$a potravní doplňky $7 D019587

650    12

$a střevní mikroflóra $x účinky léků $7 D000069196

650    _2

$a regulace genové exprese $x účinky léků $7 D005786

650    _2

$a ileum $x účinky léků $x patologie $7 D007082

650    _2

$a tenké střevo $x účinky léků $7 D007421

650    _2

$a střeva $x patologie $7 D007422

650    _2

$a lymfatické uzliny $x účinky léků $x metabolismus $7 D008198

650    _2

$a lymfocyty $x účinky léků $x metabolismus $7 D008214

650    _2

$a mužské pohlaví $7 D008297

650    _2

$a modely u zvířat $7 D023421

650    _2

$a muciny $x biosyntéza $7 D009077

650    _2

$a Panethovy buňky $x účinky léků $x metabolismus $7 D019879

650    12

$a parenterální výživa $7 D010288

650    _2

$a peptidy $x genetika $x metabolismus $7 D010455

650    _2

$a permeabilita $7 D010539

650    _2

$a fenotyp $7 D010641

650    _2

$a fylogeneze $7 D010802

650    _2

$a messenger RNA $x genetika $x metabolismus $7 D012333

650    _2

$a potkani Wistar $7 D017208

650    _2

$a proteiny těsného spoje $x metabolismus $7 D062725

655    _2

$a časopisecké články $7 D016428

700    1_

$a Heczkova, Marie $u Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague 4-140 21, Czech Republic.

700    1_

$a Dankova, Helena $u Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague 4-140 21, Czech Republic.

700    1_

$a Malinska, Hana $u Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague 4-140 21, Czech Republic.

700    1_

$a Videnska, Petra $u RECETOX, Faculty of Science, Masaryk University, Brno 625 00, Czech Republic.

700    1_

$a Vespalcova, Hana $u RECETOX, Faculty of Science, Masaryk University, Brno 625 00, Czech Republic.

700    1_

$a Micenkova, Lenka $u RECETOX, Faculty of Science, Masaryk University, Brno 625 00, Czech Republic.

700    1_

$a Bartoňová, Lenka $7 xx0248773 $u Clinical and Transplant Pathology Department, Institute for Clinical and Experimental Medicine, Prague 4-140 21, Czech Republic.

700    1_

$a Sticova, Eva $u Clinical and Transplant Pathology Department, Institute for Clinical and Experimental Medicine, Prague 4-140 21, Czech Republic.

700    1_

$a Lodererova, Alena $u Clinical and Transplant Pathology Department, Institute for Clinical and Experimental Medicine, Prague 4-140 21, Czech Republic.

700    1_

$a Prefertusová, Lucia $u Clinical and Transplant Pathology Department, Institute for Clinical and Experimental Medicine, Prague 4-140 21, Czech Republic.

700    1_

$a Sekerkova, Alena $u Prevedig, Prague 1-110 00, Czech Republic.

700    1_

$a Hradecky, Jaromir $u Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Prague, Czech Republic.

700    1_

$a Cahova, Monika $u Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague 4-140 21, Czech Republic.

773    0_

$w MED00182164 $t BioMed research international $x 2314-6141 $g Roč. 2019, č. - (2019), s. 7084734

856    41

$u https://pubmed.ncbi.nlm.nih.gov/30941370 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20191007 $b ABA008

991    __

$a 20201023113912 $b ABA008

999    __

$a ok $b bmc $g 1451328 $s 1073218

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2019 $b 2019 $c - $d 7084734 $e 20190228 $i 2314-6141 $m BioMed research international $n Biomed Res Int $x MED00182164

GRA    __

$a NV15-28745A $p MZ0

LZP    __

$a Pubmed-20191007