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The role of αβ T-cells in spontaneous regression of melanoma tumors in swine
J. Cizkova, Z. Sinkorova, K. Strnadova, M. Cervinkova, V. Horak, J. Sinkora, K. Stepanova, M. Sinkora,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Odkazy
PubMed
30308209
DOI
10.1016/j.dci.2018.10.001
Knihovny.cz E-zdroje
- MeSH
- antigeny CD4 metabolismus MeSH
- antigeny CD8 metabolismus MeSH
- cytotoxicita imunologická MeSH
- experimentální nádory imunologie MeSH
- imunofenotypizace MeSH
- kultivované buňky MeSH
- lidé MeSH
- melanom imunologie MeSH
- modely nemocí na zvířatech MeSH
- prasata imunologie MeSH
- receptory antigenů T-buněk alfa-beta metabolismus MeSH
- spontánní regrese nádoru MeSH
- T-lymfocyty imunologie MeSH
- tumor infiltrující lymfocyty imunologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Using a porcine model, we describe Melanoma-Associated CD4+CD8hi T-lymphocytes (MATL) in peripheral blood that increase during melanoma regression. These MATL possess the CD4+CD8hi phenotype and they have their direct counterparts in Tumor Infiltrating Lymphocytes (TIL) isolated from melanoma loci. Both MATL and CD4+CD8hi TIL have a similar expression of selected markers indicating that they represent effector/memory αβ T-cell subset. Moreover, although TIL also contain CD4-CD8+ T-cells, only CD4+CD8hi TIL expand during melanoma regression. Importantly, TIL isolated from different pigs and different melanoma loci among the same pig have similar composition of CD4/CD8 subsets, indicating that the composition of the MATL and TIL compartment is identical. Analysis of sorted cells from regressing pigs revealed a unique MATL subpopulation with mono-specific T-cell receptor that was further analyzed by sequencing. These results indicate that pigs regressing melanomas possess a characteristic population of recirculating T-cells playing a role in tumor control and regression.
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- $a Cizkova, Jana $u Laboratory of Applied Proteome Analyses, Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, Czech Republic; Laboratory of Tumor Biology, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czech Republic; Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic.
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- $a The role of αβ T-cells in spontaneous regression of melanoma tumors in swine / $c J. Cizkova, Z. Sinkorova, K. Strnadova, M. Cervinkova, V. Horak, J. Sinkora, K. Stepanova, M. Sinkora,
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- $a Using a porcine model, we describe Melanoma-Associated CD4+CD8hi T-lymphocytes (MATL) in peripheral blood that increase during melanoma regression. These MATL possess the CD4+CD8hi phenotype and they have their direct counterparts in Tumor Infiltrating Lymphocytes (TIL) isolated from melanoma loci. Both MATL and CD4+CD8hi TIL have a similar expression of selected markers indicating that they represent effector/memory αβ T-cell subset. Moreover, although TIL also contain CD4-CD8+ T-cells, only CD4+CD8hi TIL expand during melanoma regression. Importantly, TIL isolated from different pigs and different melanoma loci among the same pig have similar composition of CD4/CD8 subsets, indicating that the composition of the MATL and TIL compartment is identical. Analysis of sorted cells from regressing pigs revealed a unique MATL subpopulation with mono-specific T-cell receptor that was further analyzed by sequencing. These results indicate that pigs regressing melanomas possess a characteristic population of recirculating T-cells playing a role in tumor control and regression.
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- $a Sinkorova, Zuzana $u Department of Radiobiology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic. Electronic address: zuzana.sinkorova@unob.cz.
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- $a Strnadova, Kristyna $u Laboratory of Applied Proteome Analyses, Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, Czech Republic; Laboratory of Tumor Biology, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czech Republic; Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic.
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- $a Cervinkova, Monika $u Laboratory of Tumor Biology, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czech Republic; Surgical Department, 1st Medical Faculty of Charles University and Hospital Na Bulovce, Prague, Czech Republic.
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- $a Horak, Vratislav $u Laboratory of Applied Proteome Analyses, Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, Czech Republic; Laboratory of Tumor Biology, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czech Republic.
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- $a Sinkora, Jiri $u Life Sciences, Becton Dickinson Czechia, s.r.o., Prague, Czech Republic.
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- $a Sinkora, Marek $u Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, Novy Hradek, Czech Republic. Electronic address: marek@biomed.cas.cz.
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