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Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance

H. Zhang, P. Yu, J. Zhao, H. Jiang, H. Wang, Y. Zhu, MA. Botella, J. Šamaj, C. Li, J. Lin,

. 2018 ; 217 (2) : 799-812. [pub] 20171103

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19035541

Systemin (SYS), an octadecapeptide hormone processed from a 200-amino-acid precursor (prosystemin, PS), plays a central role in the systemic activation of defense genes in tomato in response to herbivore and pathogen attacks. However, whether PS mRNA is transferable and its role in systemic defense responses remain unknown. We created the transgenic tomato PS gene tagged with the green fluorescent protein (PS-GFP) using a shoot- or root-specific promoter, and the constitutive 35S promoter in Arabidopsis. Subcellular localization of PS-/SYS-GFP was observed using confocal laser scanning microscopy and gene transcripts were determined using quantitative real-time PCR. In Arabidopsis, PS protein can be processed and SYS is secreted. Shoot-/root-specific expression of PS-GFP in Arabidopsis, and grafting experiments, revealed that the PS mRNA moves in a bi-directional manner. We also found that ectopic expression of PS improves Arabidopsis resistance to the necrotrophic fungus Botrytis cinerea, consistent with substantial upregulation of the transcript levels of specific pathogen-responsive genes. Our results provide novel insights into the multifaceted mechanism of SYS signaling transport and its potential application in genetic engineering for increasing pathogen resistance across diverse plant families.

Citace poskytuje Crossref.org

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$a Zhang, Haiyan $u Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, 300387, China. Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
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$a Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance / $c H. Zhang, P. Yu, J. Zhao, H. Jiang, H. Wang, Y. Zhu, MA. Botella, J. Šamaj, C. Li, J. Lin,
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$a Yu, Pengli $u Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
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$a Jiang, Hongling $u State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
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$a Wang, Haiyang $u Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, 06411, USA.
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$a Šamaj, Jozef $u Department of Cell Biology, Centre of the Region Hana for Biotechnological and Agricultural Research, Palacky University Olomouc, Olomouc, Czech Republic.
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$a Lin, Jinxing $u Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. College of Biological Sciences, Beijing Forestry University, Beijing, 100083, China.
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