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First description in Europe of the emergence of Enterococcus faecium ST117 carrying both vanA and vanB genes, isolated in Greece
CC. Papagiannitsis, E. Malli, Z. Florou, M. Medvecky, S. Sarrou, J. Hrabak, E. Petinaki,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NV15-28663A
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
ROAD: Directory of Open Access Scholarly Resources
from 2013
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Genes, Bacterial genetics MeSH
- Bacterial Proteins genetics MeSH
- Bacteremia microbiology MeSH
- Enterococcus faecium drug effects genetics isolation & purification pathogenicity MeSH
- Vancomycin-Resistant Enterococci genetics MeSH
- Phenotype MeSH
- Genotype MeSH
- Gram-Positive Bacterial Infections microbiology MeSH
- Humans MeSH
- Carbon-Oxygen Ligases genetics MeSH
- Microbial Sensitivity Tests MeSH
- Molecular Epidemiology * MeSH
- Multilocus Sequence Typing MeSH
- Hospitals MeSH
- Plasmids genetics MeSH
- Protein Kinases genetics MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Gene Expression Regulation, Bacterial MeSH
- Vancomycin Resistance MeSH
- Teicoplanin pharmacology MeSH
- Transcription Factors genetics MeSH
- DNA Transposable Elements MeSH
- Vancomycin pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Europe MeSH
- Greece MeSH
OBJECTIVES: An Enterococcus faecium isolate (Efa-125) carrying both the vanA and vanB genes was recovered from a patient with bacteraemia treated in a Greek hospital. Since this is the first description in Europe of E. faecium carrying both vanA and vanB genes, the isolate was further studied. METHODS: Susceptibility to several antibiotics was determined using the VITEK®2 automated system. The isolate was typed by multilocus sequence typing (MLST). To define the genetic units of the vanA and vanB genes, the plasmid content of Efa-125 was analysed by pulsed-field gel electrophoresis (PFGE) of total DNA digested with S1 nuclease followed by hybridisation with digoxigenin-labelled vanA and vanB probes. In addition, plasmids and chromosomes were sequenced using the Illumina MiSeq platform. RESULTS: E. faecium Efa-125 belonged to ST117 and expressed resistance both to vancomycin and teicoplanin, with minimum inhibitory concentrations (MICs) for both of 256mg/L. The vanA gene was carried on a 29 320-bp plasmid exhibiting high similarity to pA6981 previously characterised from Enterococcus gallinarum A6981, whereas vanB was part of a Tn1549-like transposon integrated into the chromosome. Expression of the VanA phenotype was correlated with the presence of intact vanZ and vanS genes. CONCLUSIONS: This is the first detection in Greece of vanA-vanB genotype/VanA phenotype E. faecium and indicates an evolving epidemiology of vancomycin-resistant enterococci.
References provided by Crossref.org
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- $a OBJECTIVES: An Enterococcus faecium isolate (Efa-125) carrying both the vanA and vanB genes was recovered from a patient with bacteraemia treated in a Greek hospital. Since this is the first description in Europe of E. faecium carrying both vanA and vanB genes, the isolate was further studied. METHODS: Susceptibility to several antibiotics was determined using the VITEK®2 automated system. The isolate was typed by multilocus sequence typing (MLST). To define the genetic units of the vanA and vanB genes, the plasmid content of Efa-125 was analysed by pulsed-field gel electrophoresis (PFGE) of total DNA digested with S1 nuclease followed by hybridisation with digoxigenin-labelled vanA and vanB probes. In addition, plasmids and chromosomes were sequenced using the Illumina MiSeq platform. RESULTS: E. faecium Efa-125 belonged to ST117 and expressed resistance both to vancomycin and teicoplanin, with minimum inhibitory concentrations (MICs) for both of 256mg/L. The vanA gene was carried on a 29 320-bp plasmid exhibiting high similarity to pA6981 previously characterised from Enterococcus gallinarum A6981, whereas vanB was part of a Tn1549-like transposon integrated into the chromosome. Expression of the VanA phenotype was correlated with the presence of intact vanZ and vanS genes. CONCLUSIONS: This is the first detection in Greece of vanA-vanB genotype/VanA phenotype E. faecium and indicates an evolving epidemiology of vancomycin-resistant enterococci.
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