-
Je něco špatně v tomto záznamu ?
Quantitative and qualitative changes in anti-Neu5Gc antibody response following rabbit anti-thymocyte IgG induction in kidney allograft recipients
J. Rousse, A. Salama, S. Leviatan Ben-Arye, P. Hruba, J. Slatinska, G. Evanno, O. Duvaux, D. Blanchard, H. Yu, X. Chen, JM. Bach, V. Padler-Karavani, O. Viklicky, JP. Soulillou,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články
Grantová podpora
Ministry of Health, Czech Republic-conceptual development of research organization
Health-2013-INNOVATION-1-603049
FP7 Health
62466
Israeli Ministry of Science, Technology and Space
PubMed
30620396
DOI
10.1111/eci.13069
Knihovny.cz E-zdroje
- MeSH
- buněčná imunita fyziologie MeSH
- dospělí MeSH
- homologní transplantace MeSH
- imunoglobulin G farmakologie MeSH
- imunologické faktory farmakologie MeSH
- kyseliny neuraminové imunologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- prospektivní studie MeSH
- protilátky imunologie metabolismus MeSH
- průřezové studie MeSH
- senioři MeSH
- studie případů a kontrol MeSH
- thymocyty imunologie MeSH
- transplantace ledvin metody MeSH
- transplantační imunologie fyziologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Antibodies of non-human mammals are glycosylated with carbohydrate antigens, such as galactose-α-1-3-galactose (α-Gal) and N-glycolylneuraminic acid (Neu5Gc). These non-human carbohydrate antigens are highly immunogenic in humans due to loss-of-function mutations of the key genes involved in their synthesis. Such immunogenic carbohydrates are expressed on therapeutic polyclonal rabbit anti-human T-cell IgGs (anti-thymocyte globulin; ATG), the most popular induction treatment in allograft recipients. To decipher the quantitative and qualitative response against these antigens in immunosuppressed patients, particularly against Neu5Gc, which may induce endothelial inflammation in both the graft and the host. We report a prospective study of the antibody response against α-Gal and Neu5Gc-containing glycans following rabbit ATG induction compared to controls. We show a drop in the overall levels of anti-Neu5Gc antibodies at 6 and 12 months post-graft compared to the pre-existing levels due to the major early immunosuppression. However, in contrast, in a cross-sectional study there was a highly significant increase in anti-Neu5Gc IgGs levels at 6 months post-graft in the ATG-treated compared to non-treated patients(P = 0.007), with a clear hierarchy favouring anti-Neu5Gc over anti-Gal response. A sialoglycan microarray analysis revealed that the increased anti-Neu5Gc IgG response was still highly diverse against multiple different Neu5Gc-containing glycans. Furthermore, some of the ATG-treated patients developed a shift in their anti-Neu5Gc IgG repertoire compared with the baseline, recognizing different patterns of Neu5Gc-glycans. In contrast to Gal, Neu5Gc epitopes remain antigenic in severely immunosuppressed patients, who also develop an anti-Neu5Gc repertoire shift. The clinical implications of these observations are discussed.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19045061
- 003
- CZ-PrNML
- 005
- 20200113152155.0
- 007
- ta
- 008
- 200109s2019 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1111/eci.13069 $2 doi
- 035 __
- $a (PubMed)30620396
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Rousse, Juliette $u Xenothera, Nantes, France.
- 245 10
- $a Quantitative and qualitative changes in anti-Neu5Gc antibody response following rabbit anti-thymocyte IgG induction in kidney allograft recipients / $c J. Rousse, A. Salama, S. Leviatan Ben-Arye, P. Hruba, J. Slatinska, G. Evanno, O. Duvaux, D. Blanchard, H. Yu, X. Chen, JM. Bach, V. Padler-Karavani, O. Viklicky, JP. Soulillou,
- 520 9_
- $a Antibodies of non-human mammals are glycosylated with carbohydrate antigens, such as galactose-α-1-3-galactose (α-Gal) and N-glycolylneuraminic acid (Neu5Gc). These non-human carbohydrate antigens are highly immunogenic in humans due to loss-of-function mutations of the key genes involved in their synthesis. Such immunogenic carbohydrates are expressed on therapeutic polyclonal rabbit anti-human T-cell IgGs (anti-thymocyte globulin; ATG), the most popular induction treatment in allograft recipients. To decipher the quantitative and qualitative response against these antigens in immunosuppressed patients, particularly against Neu5Gc, which may induce endothelial inflammation in both the graft and the host. We report a prospective study of the antibody response against α-Gal and Neu5Gc-containing glycans following rabbit ATG induction compared to controls. We show a drop in the overall levels of anti-Neu5Gc antibodies at 6 and 12 months post-graft compared to the pre-existing levels due to the major early immunosuppression. However, in contrast, in a cross-sectional study there was a highly significant increase in anti-Neu5Gc IgGs levels at 6 months post-graft in the ATG-treated compared to non-treated patients(P = 0.007), with a clear hierarchy favouring anti-Neu5Gc over anti-Gal response. A sialoglycan microarray analysis revealed that the increased anti-Neu5Gc IgG response was still highly diverse against multiple different Neu5Gc-containing glycans. Furthermore, some of the ATG-treated patients developed a shift in their anti-Neu5Gc IgG repertoire compared with the baseline, recognizing different patterns of Neu5Gc-glycans. In contrast to Gal, Neu5Gc epitopes remain antigenic in severely immunosuppressed patients, who also develop an anti-Neu5Gc repertoire shift. The clinical implications of these observations are discussed.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a protilátky $x imunologie $x metabolismus $7 D000906
- 650 _2
- $a studie případů a kontrol $7 D016022
- 650 _2
- $a průřezové studie $7 D003430
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a buněčná imunita $x fyziologie $7 D007111
- 650 _2
- $a imunoglobulin G $x farmakologie $7 D007074
- 650 _2
- $a imunologické faktory $x farmakologie $7 D007155
- 650 _2
- $a transplantace ledvin $x metody $7 D016030
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a kyseliny neuraminové $x imunologie $7 D009438
- 650 _2
- $a prospektivní studie $7 D011446
- 650 _2
- $a thymocyty $x imunologie $7 D060168
- 650 _2
- $a transplantační imunologie $x fyziologie $7 D014181
- 650 _2
- $a homologní transplantace $7 D014184
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Salama, Apolline $u Xenothera, Nantes, France.
- 700 1_
- $a Leviatan Ben-Arye, Shani $u Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
- 700 1_
- $a Hruba, Petra $u Transplant Laboratory, Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 700 1_
- $a Slatinska, Janka $u Department of Nephrology, Transplant Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 700 1_
- $a Evanno, Gwénaëlle $u Xenothera, Nantes, France.
- 700 1_
- $a Duvaux, Odile $u Xenothera, Nantes, France.
- 700 1_
- $a Blanchard, Dominique $u Xenothera, Nantes, France.
- 700 1_
- $a Yu, Hai $u Department of Chemistry, University of California-Davis, Davis, California.
- 700 1_
- $a Chen, Xi $u Department of Chemistry, University of California-Davis, Davis, California.
- 700 1_
- $a Bach, Jean-Marie $u Immuno-Endocrinology Unit, EA4644 University/ONIRIS USC1383 INRA, Pathophysiology Department, ONIRIS-Nantes-Atlantic College of Veterinary Medicine and Food Sciences, Nantes, France.
- 700 1_
- $a Padler-Karavani, Vered $u Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
- 700 1_
- $a Viklicky, Ondrej $u Transplant Laboratory, Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Department of Nephrology, Transplant Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
- 700 1_
- $a Soulillou, Jean-Paul $u Centre de Recherche en Transplantation et Immunologie, UMR 1064, INSERM, Université de Nantes, Nantes, France. Institut de Transplantation Urologie Néphrologie (ITUN), CHU Nantes, Nantes, France.
- 773 0_
- $w MED00009632 $t European journal of clinical investigation $x 1365-2362 $g Roč. 49, č. 4 (2019), s. e13069
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/30620396 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20200109 $b ABA008
- 991 __
- $a 20200113152527 $b ABA008
- 999 __
- $a ok $b bmc $g 1483330 $s 1083734
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 49 $c 4 $d e13069 $e 20190225 $i 1365-2362 $m European journal of clinical investigation $n Eur J Clin Invest $x MED00009632
- GRA __
- $p Ministry of Health, Czech Republic-conceptual development of research organization
- GRA __
- $a Health-2013-INNOVATION-1-603049 $p FP7 Health
- GRA __
- $a 62466 $p Israeli Ministry of Science, Technology and Space
- LZP __
- $a Pubmed-20200109
