• Je něco špatně v tomto záznamu ?

Peculiar features of the plastids of the colourless alga Euglena longa and photosynthetic euglenophytes unveiled by transcriptome analyses

K. Záhonová, Z. Füssy, E. Birčák, AMG. Novák Vanclová, V. Klimeš, M. Vesteg, J. Krajčovič, M. Oborník, M. Eliáš,

. 2018 ; 8 (1) : 17012. [pub] 20181119

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19045130

Euglenophytes are a familiar algal group with green alga-derived secondary plastids, but the knowledge of euglenophyte plastid function and evolution is still highly incomplete. With this in mind we sequenced and analysed the transcriptome of the non-photosynthetic species Euglena longa. The transcriptomic data confirmed the absence of genes for the photosynthetic machinery, but provided candidate plastid-localised proteins bearing N-terminal bipartite topogenic signals (BTSs) of the characteristic euglenophyte type. Further comparative analyses including transcriptome assemblies available for photosynthetic euglenophytes enabled us to unveil salient aspects of the basic euglenophyte plastid infrastructure, such as plastidial targeting of several proteins as C-terminal translational fusions with other BTS-bearing proteins or replacement of the conventional eubacteria-derived plastidial ribosomal protein L24 by homologs of archaeo-eukaryotic origin. Strikingly, no homologs of any key component of the TOC/TIC system and the plastid division apparatus are discernible in euglenophytes, and the machinery for intraplastidial protein targeting has been simplified by the loss of the cpSRP/cpFtsY system and the SEC2 translocon. Lastly, euglenophytes proved to encode a plastid-targeted homolog of the termination factor Rho horizontally acquired from a Lambdaproteobacteria-related donor. Our study thus further documents a substantial remodelling of the euglenophyte plastid compared to its green algal progenitor.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19045130
003      
CZ-PrNML
005      
20200113081746.0
007      
ta
008      
200109s2018 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-018-35389-1 $2 doi
035    __
$a (PubMed)30451959
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Záhonová, Kristína $u Life Science Research Centre, Department of Biology and Ecology and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 701 00, Ostrava, Czech Republic.
245    10
$a Peculiar features of the plastids of the colourless alga Euglena longa and photosynthetic euglenophytes unveiled by transcriptome analyses / $c K. Záhonová, Z. Füssy, E. Birčák, AMG. Novák Vanclová, V. Klimeš, M. Vesteg, J. Krajčovič, M. Oborník, M. Eliáš,
520    9_
$a Euglenophytes are a familiar algal group with green alga-derived secondary plastids, but the knowledge of euglenophyte plastid function and evolution is still highly incomplete. With this in mind we sequenced and analysed the transcriptome of the non-photosynthetic species Euglena longa. The transcriptomic data confirmed the absence of genes for the photosynthetic machinery, but provided candidate plastid-localised proteins bearing N-terminal bipartite topogenic signals (BTSs) of the characteristic euglenophyte type. Further comparative analyses including transcriptome assemblies available for photosynthetic euglenophytes enabled us to unveil salient aspects of the basic euglenophyte plastid infrastructure, such as plastidial targeting of several proteins as C-terminal translational fusions with other BTS-bearing proteins or replacement of the conventional eubacteria-derived plastidial ribosomal protein L24 by homologs of archaeo-eukaryotic origin. Strikingly, no homologs of any key component of the TOC/TIC system and the plastid division apparatus are discernible in euglenophytes, and the machinery for intraplastidial protein targeting has been simplified by the loss of the cpSRP/cpFtsY system and the SEC2 translocon. Lastly, euglenophytes proved to encode a plastid-targeted homolog of the termination factor Rho horizontally acquired from a Lambdaproteobacteria-related donor. Our study thus further documents a substantial remodelling of the euglenophyte plastid compared to its green algal progenitor.
650    _2
$a sekvence nukleotidů $7 D001483
650    _2
$a proteiny chloroplastové $x genetika $7 D060365
650    _2
$a Euglena longa $x klasifikace $x cytologie $x genetika $7 D044802
650    12
$a molekulární evoluce $7 D019143
650    _2
$a stanovení celkové genové exprese $7 D020869
650    12
$a fotosyntéza $7 D010788
650    _2
$a fylogeneze $7 D010802
650    _2
$a plastidy $x genetika $7 D018087
650    _2
$a sekvenční homologie $7 D017385
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Füssy, Zoltán $u Institute of Parasitology, Biology Centre CAS, 370 05, České Budějovice, Czech Republic.
700    1_
$a Birčák, Erik $u Department of Genetics, Faculty of Natural Sciences, Comenius University, 842 15, Bratislava, Slovakia.
700    1_
$a Novák Vanclová, Anna M G $u Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic.
700    1_
$a Klimeš, Vladimír $u Life Science Research Centre, Department of Biology and Ecology and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 701 00, Ostrava, Czech Republic.
700    1_
$a Vesteg, Matej $u Department of Biology and Ecology, Faculty of Natural Sciences, Matej Bel University, 974 01, Banská Bystrica, Slovakia.
700    1_
$a Krajčovič, Juraj $u Department of Biology, Faculty of Natural Sciences, University of ss. Cyril and Methodius in Trnava, 917 01, Trnava, Slovakia.
700    1_
$a Oborník, Miroslav $u Institute of Parasitology, Biology Centre CAS, 370 05, České Budějovice, Czech Republic. University of South Bohemia, Faculty of Science, 370 05, České Budějovice, Czech Republic.
700    1_
$a Eliáš, Marek $u Life Science Research Centre, Department of Biology and Ecology and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 701 00, Ostrava, Czech Republic. marek.elias@osu.cz.
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 8, č. 1 (2018), s. 17012
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30451959 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200109 $b ABA008
991    __
$a 20200113082118 $b ABA008
999    __
$a ok $b bmc $g 1483399 $s 1083803
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 8 $c 1 $d 17012 $e 20181119 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
LZP    __
$a Pubmed-20200109

Najít záznam

Citační ukazatele

Možnosti archivace