-
Je něco špatně v tomto záznamu ?
Peculiar features of the plastids of the colourless alga Euglena longa and photosynthetic euglenophytes unveiled by transcriptome analyses
K. Záhonová, Z. Füssy, E. Birčák, AMG. Novák Vanclová, V. Klimeš, M. Vesteg, J. Krajčovič, M. Oborník, M. Eliáš,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2011
Free Medical Journals
od 2011
Nature Open Access
od 2011-12-01
PubMed Central
od 2011
Europe PubMed Central
od 2011
ProQuest Central
od 2011-01-01
Open Access Digital Library
od 2011-01-01
Open Access Digital Library
od 2011-01-01
Health & Medicine (ProQuest)
od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2011
Springer Nature OA/Free Journals
od 2011-12-01
- MeSH
- Euglena longa klasifikace cytologie genetika MeSH
- fotosyntéza * MeSH
- fylogeneze MeSH
- molekulární evoluce * MeSH
- plastidy genetika MeSH
- proteiny chloroplastové genetika MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie MeSH
- stanovení celkové genové exprese MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Euglenophytes are a familiar algal group with green alga-derived secondary plastids, but the knowledge of euglenophyte plastid function and evolution is still highly incomplete. With this in mind we sequenced and analysed the transcriptome of the non-photosynthetic species Euglena longa. The transcriptomic data confirmed the absence of genes for the photosynthetic machinery, but provided candidate plastid-localised proteins bearing N-terminal bipartite topogenic signals (BTSs) of the characteristic euglenophyte type. Further comparative analyses including transcriptome assemblies available for photosynthetic euglenophytes enabled us to unveil salient aspects of the basic euglenophyte plastid infrastructure, such as plastidial targeting of several proteins as C-terminal translational fusions with other BTS-bearing proteins or replacement of the conventional eubacteria-derived plastidial ribosomal protein L24 by homologs of archaeo-eukaryotic origin. Strikingly, no homologs of any key component of the TOC/TIC system and the plastid division apparatus are discernible in euglenophytes, and the machinery for intraplastidial protein targeting has been simplified by the loss of the cpSRP/cpFtsY system and the SEC2 translocon. Lastly, euglenophytes proved to encode a plastid-targeted homolog of the termination factor Rho horizontally acquired from a Lambdaproteobacteria-related donor. Our study thus further documents a substantial remodelling of the euglenophyte plastid compared to its green algal progenitor.
Department of Genetics Faculty of Natural Sciences Comenius University 842 15 Bratislava Slovakia
Department of Parasitology Faculty of Science Charles University BIOCEV Prague Czech Republic
Institute of Parasitology Biology Centre CAS 370 05 České Budějovice Czech Republic
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19045130
- 003
- CZ-PrNML
- 005
- 20200113081746.0
- 007
- ta
- 008
- 200109s2018 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1038/s41598-018-35389-1 $2 doi
- 035 __
- $a (PubMed)30451959
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Záhonová, Kristína $u Life Science Research Centre, Department of Biology and Ecology and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 701 00, Ostrava, Czech Republic.
- 245 10
- $a Peculiar features of the plastids of the colourless alga Euglena longa and photosynthetic euglenophytes unveiled by transcriptome analyses / $c K. Záhonová, Z. Füssy, E. Birčák, AMG. Novák Vanclová, V. Klimeš, M. Vesteg, J. Krajčovič, M. Oborník, M. Eliáš,
- 520 9_
- $a Euglenophytes are a familiar algal group with green alga-derived secondary plastids, but the knowledge of euglenophyte plastid function and evolution is still highly incomplete. With this in mind we sequenced and analysed the transcriptome of the non-photosynthetic species Euglena longa. The transcriptomic data confirmed the absence of genes for the photosynthetic machinery, but provided candidate plastid-localised proteins bearing N-terminal bipartite topogenic signals (BTSs) of the characteristic euglenophyte type. Further comparative analyses including transcriptome assemblies available for photosynthetic euglenophytes enabled us to unveil salient aspects of the basic euglenophyte plastid infrastructure, such as plastidial targeting of several proteins as C-terminal translational fusions with other BTS-bearing proteins or replacement of the conventional eubacteria-derived plastidial ribosomal protein L24 by homologs of archaeo-eukaryotic origin. Strikingly, no homologs of any key component of the TOC/TIC system and the plastid division apparatus are discernible in euglenophytes, and the machinery for intraplastidial protein targeting has been simplified by the loss of the cpSRP/cpFtsY system and the SEC2 translocon. Lastly, euglenophytes proved to encode a plastid-targeted homolog of the termination factor Rho horizontally acquired from a Lambdaproteobacteria-related donor. Our study thus further documents a substantial remodelling of the euglenophyte plastid compared to its green algal progenitor.
- 650 _2
- $a sekvence nukleotidů $7 D001483
- 650 _2
- $a proteiny chloroplastové $x genetika $7 D060365
- 650 _2
- $a Euglena longa $x klasifikace $x cytologie $x genetika $7 D044802
- 650 12
- $a molekulární evoluce $7 D019143
- 650 _2
- $a stanovení celkové genové exprese $7 D020869
- 650 12
- $a fotosyntéza $7 D010788
- 650 _2
- $a fylogeneze $7 D010802
- 650 _2
- $a plastidy $x genetika $7 D018087
- 650 _2
- $a sekvenční homologie $7 D017385
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Füssy, Zoltán $u Institute of Parasitology, Biology Centre CAS, 370 05, České Budějovice, Czech Republic.
- 700 1_
- $a Birčák, Erik $u Department of Genetics, Faculty of Natural Sciences, Comenius University, 842 15, Bratislava, Slovakia.
- 700 1_
- $a Novák Vanclová, Anna M G $u Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic.
- 700 1_
- $a Klimeš, Vladimír $u Life Science Research Centre, Department of Biology and Ecology and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 701 00, Ostrava, Czech Republic.
- 700 1_
- $a Vesteg, Matej $u Department of Biology and Ecology, Faculty of Natural Sciences, Matej Bel University, 974 01, Banská Bystrica, Slovakia.
- 700 1_
- $a Krajčovič, Juraj $u Department of Biology, Faculty of Natural Sciences, University of ss. Cyril and Methodius in Trnava, 917 01, Trnava, Slovakia.
- 700 1_
- $a Oborník, Miroslav $u Institute of Parasitology, Biology Centre CAS, 370 05, České Budějovice, Czech Republic. University of South Bohemia, Faculty of Science, 370 05, České Budějovice, Czech Republic.
- 700 1_
- $a Eliáš, Marek $u Life Science Research Centre, Department of Biology and Ecology and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 701 00, Ostrava, Czech Republic. marek.elias@osu.cz.
- 773 0_
- $w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 8, č. 1 (2018), s. 17012
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/30451959 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20200109 $b ABA008
- 991 __
- $a 20200113082118 $b ABA008
- 999 __
- $a ok $b bmc $g 1483399 $s 1083803
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 8 $c 1 $d 17012 $e 20181119 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
- LZP __
- $a Pubmed-20200109