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Bulky DNA adducts, microRNA profiles, and lipid biomarkers in Norwegian tunnel finishing workers occupationally exposed to diesel exhaust

I. Rynning, VM. Arlt, K. Vrbova, J. Neča, P. Rossner, J. Klema, B. Ulvestad, E. Petersen, Ø. Skare, A. Haugen, DH. Phillips, M. Machala, J. Topinka, S. Mollerup,

. 2019 ; 76 (1) : 10-16. [pub] 20181113

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19045139
E-zdroje Online Plný text

NLK ProQuest Central od 1994-01-01 do Před 6 měsíci
Nursing & Allied Health Database (ProQuest) od 1994-01-01 do Před 6 měsíci
Health & Medicine (ProQuest) od 1994-01-01 do Před 6 měsíci
Public Health Database (ProQuest) od 1994-01-01 do Před 6 měsíci

Odkazy

PubMed 30425118
DOI 10.1136/oemed-2018-105445
Knihovny.cz E-zdroje

OBJECTIVES: This study aimed to assess the biological impact of occupational exposure to diesel exhaust (DE) including DE particles (DEP) from heavy-duty diesel-powered equipment in Norwegian tunnel finishing workers (TFW). METHODS: TFW (n=69) and referents (n=69) were investigated for bulky DNA adducts (by 32P-postlabelling) and expression of microRNAs (miRNAs) (by small RNA sequencing) in peripheral blood mononuclear cells (PBMC), as well as circulating free arachidonic acid (AA) and eicosanoid profiles in plasma (by liquid chromatography-tandem mass spectrometry). RESULTS: PBMC from TFW showed significantly higher levels of DNA adducts compared with referents. Levels of DNA adducts were also related to smoking habits. Seventeen miRNAs were significantly deregulated in TFW. Several of these miRNAs are related to carcinogenesis, apoptosis and antioxidant effects. Analysis of putative miRNA-gene targets revealed deregulation of pathways associated with cancer, alterations in lipid molecules, steroid biosynthesis and cell cycle. Plasma profiles showed higher levels of free AA and 15-hydroxyeicosatetraenoic acid, and lower levels of prostaglandin D2 and 9-hydroxyoctadecadienoic acid in TFW compared with referents. CONCLUSION: Occupational exposure to DE/DEP is associated with biological alterations in TFW potentially affecting lung homoeostasis, carcinogenesis, inflammation status and the cardiovascular system. Of particular importance is the finding that tunnel finishing work is associated with an increased level of DNA adducts formation in PBMC.

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$a Arlt, Volker M $u Department of Analytical, Environmental and Forensic Sciences, MRC-PHE Centre for Environment and Health, King's College London, London, UK. NIHR Health Protection Research Unit in Health Impact of Environmental Hazards at King's College London in Partnership with Public Health England and Imperial College London, London, UK.
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$a Vrbova, Kristyna $u Department of Genetic Toxicology and Nanotoxicology, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czech Republic.
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$a Neča, Jiří $u Department of Chemistry and Toxicology, Veterinary Research Institute, Brno, Czech Republic.
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