• Je něco špatně v tomto záznamu ?

MLSB-Resistant Staphylococcus aureus in Central Greece: Rate of Resistance and Molecular Characterization

S. Sarrou, E. Malli, K. Tsilipounidaki, Z. Florou, M. Medvecky, A. Skoulakis, J. Hrabak, CC. Papagiannitsis, E. Petinaki,

. 2019 ; 25 (4) : 543-550. [pub] 20181107

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19045154

The aim of the present study was to determine the rate and mechanisms of resistance to macrolides, lincosamides, and streptogramin B (MLSB) antibiotics of Staphylococcus aureus collected in Central Greece. Of the 2,893 S. aureus collected during 2012-2017, 1,161 isolates (40.2%) exhibited resistance to at least one of the MLSB agents. The rate of erythromycin resistance was statistically significantly higher in methicillin-resistant S. aureus (MRSA) (58.6%) than in methicillin-sensitive S. aureus (MSSA) isolates (20.7%) (p = 0.002). Two hundred seventy-five representative MLSB-resistant S. aureus, including 81 MSSA and 194 MRSA isolates, were further studied. Thirty-eight MSSA isolates carried ermC, 26 MSSA were positive for ermA, whereas 17 isolates carried msrA gene. Among MRSA, the ermA gene was identified in the majority of the isolates (n = 153). Thirty-seven MRSA isolates carried ermC; three isolates carried msrA, whereas the remaining MRSA was positive for two genes (ermA and ermC). Phylogenetic analysis showed that ST225, which belongs to CC5, was the most prevalent, accounting for 137 MRSA isolates. Higher genetic diversity was found in the group of MSSA isolates, which comprised of 13 sequence types. Whole-genome sequencing data showed that all ermA-positive S. aureus, with the exception of one ST398 isolate, harbored the ermA-carrying Tn554 transposon integrated into their chromosomes. Furthermore, Illumina sequencing followed by polymerase chain reaction screening identified that ermC, which was identified in a polyclonal population of MSSA and MRSA isolates, was carried by small plasmids, like pNE131. These findings highlighted the important role of high-risk clones and of mobile elements carrying resistance genes in the successful dissemination of MLSB-resistant staphylococci.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19045154
003      
CZ-PrNML
005      
20200115131408.0
007      
ta
008      
200109s2019 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1089/mdr.2018.0259 $2 doi
035    __
$a (PubMed)30403546
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Sarrou, Stela $u 1 Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
245    10
$a MLSB-Resistant Staphylococcus aureus in Central Greece: Rate of Resistance and Molecular Characterization / $c S. Sarrou, E. Malli, K. Tsilipounidaki, Z. Florou, M. Medvecky, A. Skoulakis, J. Hrabak, CC. Papagiannitsis, E. Petinaki,
520    9_
$a The aim of the present study was to determine the rate and mechanisms of resistance to macrolides, lincosamides, and streptogramin B (MLSB) antibiotics of Staphylococcus aureus collected in Central Greece. Of the 2,893 S. aureus collected during 2012-2017, 1,161 isolates (40.2%) exhibited resistance to at least one of the MLSB agents. The rate of erythromycin resistance was statistically significantly higher in methicillin-resistant S. aureus (MRSA) (58.6%) than in methicillin-sensitive S. aureus (MSSA) isolates (20.7%) (p = 0.002). Two hundred seventy-five representative MLSB-resistant S. aureus, including 81 MSSA and 194 MRSA isolates, were further studied. Thirty-eight MSSA isolates carried ermC, 26 MSSA were positive for ermA, whereas 17 isolates carried msrA gene. Among MRSA, the ermA gene was identified in the majority of the isolates (n = 153). Thirty-seven MRSA isolates carried ermC; three isolates carried msrA, whereas the remaining MRSA was positive for two genes (ermA and ermC). Phylogenetic analysis showed that ST225, which belongs to CC5, was the most prevalent, accounting for 137 MRSA isolates. Higher genetic diversity was found in the group of MSSA isolates, which comprised of 13 sequence types. Whole-genome sequencing data showed that all ermA-positive S. aureus, with the exception of one ST398 isolate, harbored the ermA-carrying Tn554 transposon integrated into their chromosomes. Furthermore, Illumina sequencing followed by polymerase chain reaction screening identified that ermC, which was identified in a polyclonal population of MSSA and MRSA isolates, was carried by small plasmids, like pNE131. These findings highlighted the important role of high-risk clones and of mobile elements carrying resistance genes in the successful dissemination of MLSB-resistant staphylococci.
650    _2
$a antibakteriální látky $x farmakologie $7 D000900
650    _2
$a bakteriální proteiny $x genetika $7 D001426
650    _2
$a mnohočetná bakteriální léková rezistence $x genetika $7 D024901
650    _2
$a genotyp $7 D005838
650    _2
$a lidé $7 D006801
650    _2
$a linkosamidy $x farmakologie $7 D055231
650    _2
$a makrolidy $x farmakologie $7 D018942
650    _2
$a methicilin rezistentní Staphylococcus aureus $x účinky léků $x genetika $7 D055624
650    _2
$a mikrobiální testy citlivosti $x metody $7 D008826
650    _2
$a fylogeneze $7 D010802
650    _2
$a stafylokokové infekce $x farmakoterapie $x mikrobiologie $7 D013203
650    _2
$a streptogramin B $x farmakologie $7 D025381
651    _2
$a Řecko $7 D006115
655    _2
$a časopisecké články $7 D016428
700    1_
$a Malli, Ergina $u 1 Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
700    1_
$a Tsilipounidaki, Katerina $u 1 Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
700    1_
$a Florou, Zoi $u 1 Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
700    1_
$a Medvecky, Matej $u 2 Veterinary Research Institute, Brno, Czech Republic. 3 Faculty of Science, National Center for Biomolecular Research, Masaryk University, Brno, Czech Republic.
700    1_
$a Skoulakis, Anargyros $u 1 Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
700    1_
$a Hrabak, Jaroslav $u 4 Faculty of Medicine, Biomedical Center, Charles University, Pilsen, Czech Republic.
700    1_
$a Papagiannitsis, Costas C $u 1 Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
700    1_
$a Petinaki, Efi $u 1 Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
773    0_
$w MED00008749 $t Microbial drug resistance (Larchmont, N.Y.) $x 1931-8448 $g Roč. 25, č. 4 (2019), s. 543-550
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30403546 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200109 $b ABA008
991    __
$a 20200115131741 $b ABA008
999    __
$a ok $b bmc $g 1483423 $s 1083827
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 25 $c 4 $d 543-550 $e 20181107 $i 1931-8448 $m Microbial drug resistance $n Microb Drug Resist $x MED00008749
LZP    __
$a Pubmed-20200109

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace