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Dynamics of Beta-Glucosidase Zm-p60.1 Ectopic Expression During Transgenic Pollen Development: A Histochemical Approach

J Dubová, P Mlejnek, B Brzobohatý

. 1999 ; 18 (Suppl. 1) : 112-116.
. 1999 ; 18 (Suppl. 1) : 112-116.

Jazyk angličtina Země Slovensko

Perzistentní odkaz   https://www.medvik.cz/link/bmc19048208
E-zdroje Online

NLK Free Medical Journals od 1982 do 2006

Zm-p60.1 is maize cDNA coding cytokinin-glucoside specific beta-glucosidase. Indigogenic method was used for histochemical localization of Zm-p60.1 beta-glucosidase activity in various developmental stages of transgenic tobacco anthers. Expression of Zm-p60.1 cDNA in T7 tobacco plants is controlled by the CaMV 35S promoter. Another type of tobacco transformant expresses Zm-p60.1 under the control of LAT 52 promoter. Histochemical detection has proved different patterns of beta-glucosidase activity during tobacco pollen development in these two types of transformants. Zm-p60.1 beta-glucosidase activity had not direct influence on pollen germinability.

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