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Dynamics of Beta-Glucosidase Zm-p60.1 Ectopic Expression During Transgenic Pollen Development: A Histochemical Approach
J Dubová, P Mlejnek, B Brzobohatý
Jazyk angličtina Země Slovensko
NLK
Free Medical Journals
od 1982 do 2006
- MeSH
- beta-glukosidasa genetika MeSH
- DNA primery genetika MeSH
- exprese genu MeSH
- geneticky modifikované rostliny MeSH
- jedovaté rostliny MeSH
- kukuřice setá enzymologie genetika růst a vývoj MeSH
- messenger RNA genetika metabolismus MeSH
- pyl enzymologie genetika růst a vývoj MeSH
- RNA rostlin genetika metabolismus MeSH
- sekvence nukleotidů MeSH
- tabák MeSH
- transformace genetická MeSH
Zm-p60.1 is maize cDNA coding cytokinin-glucoside specific beta-glucosidase. Indigogenic method was used for histochemical localization of Zm-p60.1 beta-glucosidase activity in various developmental stages of transgenic tobacco anthers. Expression of Zm-p60.1 cDNA in T7 tobacco plants is controlled by the CaMV 35S promoter. Another type of tobacco transformant expresses Zm-p60.1 under the control of LAT 52 promoter. Histochemical detection has proved different patterns of beta-glucosidase activity during tobacco pollen development in these two types of transformants. Zm-p60.1 beta-glucosidase activity had not direct influence on pollen germinability.
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