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Analysis of differential expression of neurotransmitter receptors with TNF-a treatment in rat primary hypothalamic cell
Ting Lu, Jiu-cheng Xia, Ting Long, Da-nian Qin
Jazyk angličtina Země Česko
Typ dokumentu práce podpořená grantem
- MeSH
- hypothalamus účinky léků MeSH
- krysa rodu rattus MeSH
- polymerázová řetězová reakce metody MeSH
- receptory neurotransmiterů * klasifikace účinky léků MeSH
- regulace genové exprese účinky léků MeSH
- TNF-alfa * MeSH
- Check Tag
- krysa rodu rattus MeSH
- Publikační typ
- práce podpořená grantem MeSH
In this study, tumor necrosis factor-alpha (TNF-α) was incubated to primary hypothalamic cell culture of rat as an important inflammatory cytokine and the effect of TNF-α to neurotransmitter receptors of the hypothalamus in vitro by real-time fluorescence quantitative PCR with PCR array for researching the effect of TNF-α to neuroendocrine system was analyzed. The result showed that the expression level of Avpr1a, Gabrr1, Grin1, Htr4, and Tspo 5 genes was obviously up and the other Adrb2, Cckbr, Chrm5, Chrna6, Gabra5, Gabra6, Grm8, Htr2c, Htr3a, Sstr1, and Tacr1 11 genes were down. Especially, Grin1 and Tacr1 were affected obviously, increased 3.12 and decreased 2.76, respectively. So, TNF-α can modulate the expression of many receptors of neurotransmitters of the hypothalamus by the special cell signal transduction pathways and alter the responsibility and exciting of these neurons to neurotransmitters corresponding. Furthermore, these changes can affect many hormone secretion of the hypothalamus and affect neuroendocrine function of rat.
Department of Bio chemical Engineering Panzhihua University Panzhihua China
Department of Physiology Medical School Shantou University Shantou China
Citace poskytuje Crossref.org
Literatura
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- $a In this study, tumor necrosis factor-alpha (TNF-α) was incubated to primary hypothalamic cell culture of rat as an important inflammatory cytokine and the effect of TNF-α to neurotransmitter receptors of the hypothalamus in vitro by real-time fluorescence quantitative PCR with PCR array for researching the effect of TNF-α to neuroendocrine system was analyzed. The result showed that the expression level of Avpr1a, Gabrr1, Grin1, Htr4, and Tspo 5 genes was obviously up and the other Adrb2, Cckbr, Chrm5, Chrna6, Gabra5, Gabra6, Grm8, Htr2c, Htr3a, Sstr1, and Tacr1 11 genes were down. Especially, Grin1 and Tacr1 were affected obviously, increased 3.12 and decreased 2.76, respectively. So, TNF-α can modulate the expression of many receptors of neurotransmitters of the hypothalamus by the special cell signal transduction pathways and alter the responsibility and exciting of these neurons to neurotransmitters corresponding. Furthermore, these changes can affect many hormone secretion of the hypothalamus and affect neuroendocrine function of rat.
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