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Serum proteomic analysis of melanoma patients with immunohistochemical profiling of primary melanomas and cultured cells: Pilot study
J. Kučera, K. Strnadová, B. Dvořánková, L. Lacina, I. Krajsová, J. Štork, H. Kovářová, HK. Skalníková, P. Vodička, J. Motlík, P. Dundr, K. Smetana, O. Kodet,
Jazyk angličtina Země Řecko
Typ dokumentu srovnávací studie, časopisecké články
Grantová podpora
NV16-30954A
MZ0
CEP - Centrální evidence projektů
NV16-30954A
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Plný text - Článek
NLK
Free Medical Journals
od 2006 do Před 1 rokem
ProQuest Central
od 2012-01-01
Medline Complete (EBSCOhost)
od 2014-06-01
Health & Medicine (ProQuest)
od 2012-01-01
PubMed
31545456
DOI
10.3892/or.2019.7319
Knihovny.cz E-zdroje
- MeSH
- chemokiny krev MeSH
- dospělí MeSH
- fibroblasty asociované s nádorem metabolismus MeSH
- krevní proteiny analýza MeSH
- lidé středního věku MeSH
- lidé MeSH
- melanom krev metabolismus MeSH
- nádorové biomarkery krev MeSH
- nádorové buněčné linie MeSH
- pilotní projekty MeSH
- prognóza MeSH
- proteomika metody MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
The steadily increasing incidence of malignant melanoma (MM) and its aggressive behaviour makes this tumour an attractive cancer research topic. The tumour microenvironment is being increasingly recognised as a key factor in cancer biology, with an impact on proliferation, invasion, angiogenesis and metastatic spread, as well as acquired therapy resistance. Multiple bioactive molecules playing cooperative roles promote the chronic inflammatory milieu in tumours, making inflammation a hallmark of cancer. This specific inflammatory setting is evident in the affected tissue. However, certain mediators can leak into the systemic circulation and affect the whole organism. The present study analysed the complex inflammatory response in the sera of patients with MM of various stages. Multiplexed proteomic analysis (Luminex Corporation) of 31 serum proteins was employed. These targets were observed in immunohistochemical profiles of primary tumours from the same patients. Furthermore, these proteins were analysed in MM cell lines and the principal cell population of the melanoma microenvironment, cancer‑associated fibroblasts. Growth factors such as hepatocyte growth factor, granulocyte‑colony stimulating factor and vascular endothelial growth factor, chemokines RANTES and interleukin (IL)‑8, and cytokines IL‑6, interferon‑α and IL‑1 receptor antagonist significantly differed in these patients compared with the healthy controls. Taken together, the results presented here depict the inflammatory landscape that is altered in melanoma patients, and highlight potentially relevant targets for therapy improvement.
Institute of Anatomy 1st Faculty of Medicine Charles University Prague 128 00 Czech Republic
Institute of Pathology 1st Faculty of Medicine Charles University Prague 128 00 Czech Republic
Citace poskytuje Crossref.org
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