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Imaging of Mouse Brain Fixated in Ethanol in Micro-CT

J. Mrzílková, M. Patzelt, P. Gallina, Z. Wurst, M. Šeremeta, J. Dudák, F. Krejčí, J. Žemlička, V. Musil, J. Karch, J. Rosina, P. Zach,

. 2019 ; 2019 (-) : 2054262. [pub] 20190714

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc20006063

Micro-CT imaging is a well-established morphological method for the visualization of animal models. We used ethanol fixation of the mouse brains to perform high-resolution micro-CT scans showing in great details brain grey and white matters. It was possible to identify more than 50 neuroanatomical structures on the 5 selected coronal sections. Among white matter structures, we identified fornix, medial lemniscus, crossed tectospinal pathway, mammillothalamic tract, and the sensory root of the trigeminal ganglion. Among grey matter structures, we identified basal nuclei, habenular complex, thalamic nuclei, amygdala, subparts of hippocampal formation, superior colliculi, Edinger-Westphal nucleus, and others. We suggest that micro-CT of the mouse brain could be used for neurohistological lesions evaluation as an alternative to classical neurohistology because it does not destroy brain tissue.

Citace poskytuje Crossref.org

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$a Micro-CT imaging is a well-established morphological method for the visualization of animal models. We used ethanol fixation of the mouse brains to perform high-resolution micro-CT scans showing in great details brain grey and white matters. It was possible to identify more than 50 neuroanatomical structures on the 5 selected coronal sections. Among white matter structures, we identified fornix, medial lemniscus, crossed tectospinal pathway, mammillothalamic tract, and the sensory root of the trigeminal ganglion. Among grey matter structures, we identified basal nuclei, habenular complex, thalamic nuclei, amygdala, subparts of hippocampal formation, superior colliculi, Edinger-Westphal nucleus, and others. We suggest that micro-CT of the mouse brain could be used for neurohistological lesions evaluation as an alternative to classical neurohistology because it does not destroy brain tissue.
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$a Patzelt, Matěj $u Specialized Laboratory of Experimental Imaging Third Faculty of Medicine, Charles University, Institute of Experimental and Applied Physics and Faculty of Biomedical Engineering, Czech Technical University in Prague, Prague, Czech Republic. Department of Anatomy, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
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$a Gallina, Pasquale $u Department of Surgery and Translational Medicine, Neurosurgery Unit, Florence School of Neurosurgery, University of Florence, Florence, Italy.
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$a Šeremeta, Martin $u Department of Anatomy, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
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$a Dudák, Jan $u Institute of Experimental and Applied Physics, Czech Technical University, Prague, Czech Republic. Czech Technical University in Prague, Faculty of Biomedical Engineering, Kladno, Czech Republic.
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$a Krejčí, František $u Institute of Experimental and Applied Physics, Czech Technical University, Prague, Czech Republic.
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$a Karch, Jakub $u Institute of Experimental and Applied Physics, Czech Technical University, Prague, Czech Republic. $7 xx0273614
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$a Rosina, Jozef $u Czech Technical University in Prague, Faculty of Biomedical Engineering, Kladno, Czech Republic. Department of Medical Biophysics and Informatics, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
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$a Zach, Petr $u Specialized Laboratory of Experimental Imaging Third Faculty of Medicine, Charles University, Institute of Experimental and Applied Physics and Faculty of Biomedical Engineering, Czech Technical University in Prague, Prague, Czech Republic. Department of Anatomy, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
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