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Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples
J. Lačná, J. Přikryl, N. Teshima, H. Murakami, Y. Esaka, F. Foret, P. Kubáň,
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
17-31945A
Ministry of Health of the Czech Republic - International
RVO:68081715
Czech Academy of Sciences - International
LQ1601
Central European Institute of Technology - International
RVO:68081715
Akademie Věd České Republiky - International
17-31945A
Ministerstvo Zdravotnictví Ceské Republiky - International
NV17-31945A
MZ0
CEP Register
Digital library NLK
Full text - Article
NLK
Medline Complete (EBSCOhost)
from 2012-10-01 to 1 year ago
- MeSH
- Breath Tests methods MeSH
- Equipment Design MeSH
- Electric Conductivity MeSH
- Electrophoresis, Capillary methods MeSH
- Spectrometry, Fluorescence methods MeSH
- Glutathione analysis isolation & purification MeSH
- Ions analysis isolation & purification MeSH
- Carboxylic Acids analysis isolation & purification MeSH
- Humans MeSH
- Limit of Detection MeSH
- Linear Models MeSH
- Reproducibility of Results MeSH
- Tears chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual C4 D-LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 15 mM 2-amino-2-hydroxymethyl-propane-1,3-diol, and 2 mM 18-crown-6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double-opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using C4 D and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGE may be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.
References provided by Crossref.org
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- $a Lačná, Júlia $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Brno, Czech Republic. Department of Chemistry, Masaryk University, Brno, Czech Republic.
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- $a Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples / $c J. Lačná, J. Přikryl, N. Teshima, H. Murakami, Y. Esaka, F. Foret, P. Kubáň,
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- $a In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual C4 D-LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 15 mM 2-amino-2-hydroxymethyl-propane-1,3-diol, and 2 mM 18-crown-6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double-opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using C4 D and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGE may be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.
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- $a Kubáň, Petr $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Brno, Czech Republic. Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic.
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