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Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

J. Lačná, J. Přikryl, N. Teshima, H. Murakami, Y. Esaka, F. Foret, P. Kubáň,

. 2019 ; 40 (18-19) : 2390-2397. [pub] 20190626

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20006234

Grantová podpora
17-31945A Ministry of Health of the Czech Republic - International
RVO:68081715 Czech Academy of Sciences - International
LQ1601 Central European Institute of Technology - International
RVO:68081715 Akademie Věd České Republiky - International
17-31945A Ministerstvo Zdravotnictví Ceské Republiky - International
NV17-31945A MZ0 CEP - Centrální evidence projektů

In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual C4 D-LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 15 mM 2-amino-2-hydroxymethyl-propane-1,3-diol, and 2 mM 18-crown-6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double-opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using C4 D and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGE may be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.

Citace poskytuje Crossref.org

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