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Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells
LC. Noack, P. Pejchar, J. Sekereš, Y. Jaillais, M. Potocký,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- exprese genu MeSH
- fluorescenční barviva analýza metabolismus MeSH
- fluorescenční mikroskopie metody MeSH
- fosfatidylinositoly analýza metabolismus MeSH
- fosfolipidy analýza metabolismus MeSH
- konfokální mikroskopie metody MeSH
- luminescentní proteiny analýza genetika MeSH
- pyl chemie genetika MeSH
- rostlinné buňky chemie metabolismus MeSH
- tabák chemie cytologie genetika MeSH
- transformace genetická MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Anionic phospholipids represent only minor fraction of cell membranes lipids but they are critically important for many membrane-related processes, including membrane identity, charge, shape, the generation of second messengers, and the recruitment of peripheral proteins. The main anionic phospholipids of the plasma membrane are phosphoinositides phosphatidylinositol 4-phosphate (PI4P), phosphatidylinositol 4,5-bisphosphate (PI4,5P2), phosphatidylserine (PS), and phosphatidic acid (PA). Recent insights in the understanding of the nature of protein-phospholipid interactions enabled the design of genetically encoded fluorescent molecular probes that can interact with various phospholipids in a specific manner allowing their imaging in live cells. Here, we describe the use of transiently transformed plant cells to study phospholipid-dependent membrane recruitment.
Citace poskytuje Crossref.org
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- $a Noack, Lise C $u Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, CNRS, INRA, Lyon, France.
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- $a Anionic phospholipids represent only minor fraction of cell membranes lipids but they are critically important for many membrane-related processes, including membrane identity, charge, shape, the generation of second messengers, and the recruitment of peripheral proteins. The main anionic phospholipids of the plasma membrane are phosphoinositides phosphatidylinositol 4-phosphate (PI4P), phosphatidylinositol 4,5-bisphosphate (PI4,5P2), phosphatidylserine (PS), and phosphatidic acid (PA). Recent insights in the understanding of the nature of protein-phospholipid interactions enabled the design of genetically encoded fluorescent molecular probes that can interact with various phospholipids in a specific manner allowing their imaging in live cells. Here, we describe the use of transiently transformed plant cells to study phospholipid-dependent membrane recruitment.
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- $a Pejchar, Přemysl $u Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech Republic. Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.
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- $a Potocký, Martin $u Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech Republic. potocky@ueb.cas.cz. Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic. potocky@ueb.cas.cz.
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